Instrumentation
Zeiss LSM 880 NLO Laser Scanning Microscope
Laser scanning microscope:
o 355nm UV laser
o 405nm, 594nm, 561nm and 633nm diode lasers
o Argon laser (458nm, 488nm and 514nm lines)
Coherent Chameleon Vision II tunable (680nm – 1080nm) IR multiphoton laser system
Epifluorescence sample preview:
o DAPI, CFP, GFP, YFP, Rhodamine, and mCherry filters
o Qdot 565, 585, 605, and 655 filters
63x oil-immersion and 40x water-immersion objectives
Definite focus and Motorized scanning stage
Heated incubation chamber with CO2 and humidity control for live cell imaging
ZEN imaging software with FCS and photobleaching modules
Yokogawa CSU-W1 Spinning Disk Confocal on Nikon T2i Eclipse Microscope
Confocal imaging with 405nm, 488nm, 561nm and 640nm lasers
Epifluorescence wide-filed imaging with Lumencor Sola lightsource:
o DAPI, FITC, Cy3 and Cy5 channels
CoolLED 320/380nm lightsource for Fura-2 calcium imaging
Two Hamamatsu Orca Flash4 sCMOS cameras
Fully automated scanning stage for tile imaging:
o Fast confocal acquisition of large area mosaic images
o Automated multi-point imaging
PI piezoelectric Z-stage:
o Fast confocal acquisition of multiple Z-stacks
PFS focus stabilization and NIS imaging software
Zeiss AxioObserver Z1 Inverted Widefield Microscope
Epifluorescence imaging
o DAPI, CFP, GFP, YFP, Cy3, Texas Red, Cy5 and Cy7 filters
o Hamamatsu ORCA Flash 4.0 LT sCMOS monochrome camera
Brightfield transmitted light imaging
o Brightfield, DIC and Phase contrast
o AxioCam MRc5 color CCD camera