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ZEISS Elyra 7 with Lattice SIM²

Type

  • Widefield structured illumination microscope
  • Capabilities

    • Super resolution 2D and 3D imaging of live or fixed cells
    • Apotome SIM: 170 nm lateral, 450 nm axial
    • Apotome SIM2: 140 nm lateral, 300 nm axial
    • Lattice SIM: 120 nm lateral, 300 nm axial
    • Lattice SIM2: 60 nm lateral, 200 nm axial
    • Simultaneous 4 color acquisition (2 colors/camera)

    Laser Lines

    • 405 nm, 458 nm, 561 nm, 642 nm

    Objectives (Apotome)

    • 10x, dry, 0.30 NA, 5.2 mm
    • 20x, dry, 0.80 NA, 0.55 mm
    • 40x, water, 1.2 NA, 0.28 mm
    • 40x, oil, 1.4 NA, 0.13 mm, DIC

    Objectives (SIM)

    • 63x, oil, 1.4 NA, 0.19 mm, DIC
    • 63x, oil, 1.46 NA, 0.1 mm

    Detectors

    • 2 pco.edge sCMOS cameras

    Zeiss LSM 880 Airyscan

    Type

    Laser scanning confocal microscope

    Capabilities

    • Super-resolution confocal imaging – resolution of 140nm, xy and 350nm, z in fixed and live 2D/3D cells
    • Improved sensitivity imaging (Improved signal to noise) – 4x to 8x more sensitive than Zeiss LSM 780 in fixed and live 2D/3D cells
    • Faster imaging – 27 frames per second at 480 x 480 pixels in fixed and live cells
    • Fluorescent Correlation Spectroscopy (FCS)

    Laser Lines

    • 405, 458, 488, 514, 561, 594, 633 nm

    Objectives

    • 10x, dry, 0.45NA, 2.0 mm
    • 20x, dry, 0.80NA, 0.55 mm
    • 40x, water, 1.2NA, 0.28 mm
    • 40x, oil, 1.3NA, 0.21 mm
    • 63x, oil, 1.4NA, 0.19 mm
    • 63x, multi-immersion, 1.2NA, 0.49 mm
    • 20x, dry, 0.80NA, 0.55 mm
    • 40x, water, 1.2NA, 0.28 mm
    • 40x, oil, 1.3NA, 0.21 mm
    • 63x, oil, 1.4NA, 0.19 mm
    • 63x, multi-immersion, 1.2NA, 0.49 mm

    Detectors

    • (2) Multialkali photomultiplier tube (MA-PMT)
    • Spectral Detector – 32 Channel Gallium Arsenide Phosphide Photomultiplier Tube (GaAsP PMT)
    • Airyscan detector – array of 32 individual GaAsP-PMT elements that act as a pinhole size of 0.2 Airy units (AU)

    Leica SP8 with Digital Lightsheet

    Type

    Laser scanning confocal with digital lightsheet microscope

    Capabilities

    • 2D / 3D imaging of fixed or live fluorescence-labeled samples
    • Imaging of interactions and dynamics of fluorescence labeled molecules
    • Digital light sheet imaging of cleared large 3D samples both fixed and live
    • Spectral Imaging
    • Forster Resonance Energy Transfer (FRET)
    • Fluorescence Recovery After Photobleaching (FRAP)
    • Laser Lines

    Laser Lines

    • 405, 488, 552, 638 nm

    Objectives

    • 1.6x, dry, 0.05NA*
    • 2.5x, dry, 0.07NA, 9.2 mm*
    • 5x, dry, 0.15NA, 12 mm
    • 5x, multi-immersion, 0.15NA#
    • 10x, dry, 0.4NA, 2.2 mm*
    • 10x, water, 0.3NA, 3.6 mm#
    • 20x, multi-immersion, 0.75NA, 0.67 mm
    • 25x, water, 0.95NA, 2.5 mm#
    • 40x, dry, 0.85NA, 0.21 mm
    • 63x, water, 1.2NA, 0.30 mm,
    • 63x, oil, 1.4NA, 0.14 mm
    • 100x, oil, 1.4NA, 0.13 mm
    • *Illumination objectives for Digital Lightsheet
    • #Detection objectives for Digital Lightsheet
    • TwinFlect Mirrors for Digital Lightsheet
    • 7.8 mm width; Sample Immersion Liquid – Glycerol, Water; Illumination Obj. – 1.6x, dry; Detection Obj. 5x, multi-immersion or 10x, water
    • 5 mm width; Sample Immersion Liquid -Water; Illumination Obj. – 2.5x, dry; Detection Obj. 10x, water or 25x, water
    • 2.5 mm width; Sample Immersion Liquid -Water; Illumination Obj. – 2.5x, dry or 10x, dry ; Detection Obj. 10x, water or 25x, water

    Detectors

    • (1) Photomultiplier tube
    • (2) Hybrid detectors (HyD)

    Instructions

    Andor spinning disk confocol on Leica DMi8 base

    Type

    Spinning disk confocal

    Capabilities

    • 2D / 3D imaging of fixed or live fluorescence-labeled samples
    • Imaging of interactions and dynamics of fluorescence labeled molecules
    • Large area montages

    Laser Lines

    • 405, 445, 488, 514, 561, 594, 640, 730 nm

    Objectives

    • 1.6x, dry, 0.05NA
    • 5x, dry, 0.15NA, 12 mm
    • 10x, dry, 0.4NA, 2.2 mm
    • 20x, dry, 0.75NA, 0.62 mm
    • 63x, water, 1.2NA, 0.30 mm,
    • 63x, oil, 1.4NA, 0.14 mm

    Detectors

    • Andor Xyla sCMOS camera

    Zeiss LSM710

    Type

    Laser scanning confocal microscope

    Capabilities

    • 2D / 3D imaging of fixed or live fluorescence-labeled samples
    • Imaging of interactions and dynamics of fluorescence labeled molecules

    Laser Lines

    • 405, 458, 488, 514, 561, 594, 633, 690 – 1000 nm (pulsed)

    Objectives

    • 5X, dry, 0.15 NA, 13.6 mm
    • 10X, dry, 0.45 NA, 2.00 mm
    • 10X, water, 0.45 NA, 1.8 mm
    • 20X, dry, 0.4 NA, 7.9 mm
    • 40X, water, 1.1 NA, 0.62 mm
    • 63X, oil, 1.4 NA, 0.19 mm

    Detectors

    • Photomultiplier tubes
    • Spectral detector

    Instructions

    Zeiss LSM780

    Type

    Laser scanning confocal microscope

    Capabilities

    • 2D / 3D imaging of fixed or live fluorescence-labeled samples
    • Imaging of interactions and dynamics of fluorescence labeled molecules
    • Live animal imaging

    Laser Lines

    • 405, 458, 488, 514, 561, 633, 690 – 1000 nm (pulsed)

    Objectives

    • 10X, dry, 0.3 NA, 5.1 mm
    • 20X, dry, 0.8 NA, 0.55 mm
    • 20X multi-immersion
    • 20X water (dipping lens, not on turret), 1.0NA, 0.19 mm
    • 40X, water, 1.1 NA, 0.28 mm
    • 40X, oil, 1.3 NA, 0.2 mm
    • 63X, oil, 1.4 NA, 0.19 mm

    Detectors

    • Photomultiplier tubes
    • Spectral detector
    • Non-descanned detectors (for use with 2P laser)
    • Fluorescence life-time detectors

    Instructions

    Nikon STORM

    Type

    Widefield super-resolution microscope for fixed cells only

    Capabilities

    • Super-resolution by Point Localization Microscopy – resolution 20nm in xy and 50nm in z.
    • Recommended modality is dSTORM with CF-647 dye (improved version of Alexa Fluor 647 dye).

    Laser Lines

    • 405, 488, 561, 647 nm

    Objectives

    • 100x oil, 1.49 NA, 0.13 mm WD
    • 100x silicon oil, 1.35 NA, 0.3 mm WD

    Cameras

    • Andor iXON DU-897U

    Nikon SIM

    Type

    Widefield super-resolution microscope for fixed cells only

    Capabilities

    • N-SIM: Super-resolution Structural Illumination Microscopy (SIM) – resolution of 110nm in xy and 275nm in z.  Compatible with conventional immunofluorescent staining.
    • “External-phase” trans-illumination imaging (on side eye-port camera), which is useful for correlative light/electron microscopy.  None of the high NA objectives on this microscope are compatible with regular phase imaging.

    Laser Lines

    • 405, 488, 561, 640 nm

    Objectives

    • 10x, dry, 0.3 NA, 16 mm WD
    • 60x water, 1.27 NA, 0.17 mm WD
    • 100x oil, 1.49 NA, 0.13 mm WD

    Cameras

    • Andor iXON DU-897E for SIM
    • Nikon DS-QiMC for external phase microscopy

    Nikon Multifunctional Microscope (MFM)

    Type

    Widefield

    Capabilities

    • 2D / 3D imaging of fixed or live fluorescence-labeled samples
    • High content analysis
    • TIRF
    • Polarization imaging
    • Simultaneous 2-color imaging
    • Single molecule imaging

    Excitation lines

    • (LED illuminator)
    • 395, 440, 470, 508, 555, 640 nm

    Objectives

    • 4X, dry, 0.13 NA, 16.5 mm
    • 20x, dry, 0.75 NA, 1.00 mm
    • 60X, oil, 1.40 NA, 0.13 mm
    • 100X, oil, 1.45 NA, 0.13 mm

    Detectors

    • Andor NeoZyla sCMOS camera
    • Andor iXon Ultra EMCCD camera

    Asylum Research Cypher-VRS

    Type

    Atomic force microscope

    Capabilities

    • Video-rate scanning for molecular dynamics imaging
    • Contact and tapping mode imaging in air and liquid environments
    • Force spectroscopy
    • Mechanical mapping
    • Temperature control
    • Perfusion chamber
    • Semi-automated probe alignment and calibration
    • Built-in analysis tools

    Objectives

    • 20X reflective objective for cantilever setup and sample locating

    Sample formats

    • Standard imaging: 10 mm diameter substrate
    • Video-rate imaging: 3 mm diameter substrate

    Celigo

    Type

    Plate reading microscope

    Capabilities

    • Built in analysis tools
    • Fast, widefield imaging of entire well for multiwell plate
    • Built in protocols for live/dead counting, growth curves, spheroid analysis etc.

    Excitation/Emission filters

    • Blue 377/477
    • Green 483/536
    • Red 531/629
    • Far Red 632/692
    • Brightfield

    Objectives

    • 1 objective optimized for entire well imaging & built in analysis features

    Sample formats

    • Works best with supported/recommended plates
    • Microwell plates: 6, 12, 24, 48, 96, 384, 1536 wells
    • Slides
    • 10 cm Petri Dish
    • T25 Flasks

    Instructions