Instrumentation

Zeiss LSM 880 NLO Laser Scanning Microscope

  • Laser scanning microscope:
    • 355nm UV laser
    • 405nm, 594nm, 561nm and 633nm diode lasers
    • Argon laser (458nm, 488nm and 514nm lines)
  • Coherent Chameleon Vision II tunable (680nm – 1080nm) IR multiphoton laser system
  • Epifluorescence sample preview:
    • DAPI, CFP, GFP, YFP, Rhodamine, and mCherry filters
    • Qdot 565, 585, 605, and 655 filters
  • 63x oil-immersion and 40x water-immersion objectives
  • Definite focus and Motorized scanning stage
  • Heated incubation chamber with CO2 and humidity control for live cell imaging
  • ZEN imaging software with FCS and photobleaching modules

Yokogawa CSU-W1 Spinning Disk Confocal on Nikon T2i Eclipse Microscope

  • Confocal imaging with 405nm, 488nm, 561nm and 640nm lasers
  • Epifluorescence wide-filed imaging with Lumencor Sola lightsource:
    • DAPI, FITC, Cy3 and Cy5 channels
  • CoolLED 320/380nm lightsource for Fura-2 calcium imaging
  • Two Hamamatsu Orca Flash4 sCMOS cameras
  • Fully automated scanning stage for tile imaging:
    • Fast confocal acquisition of large area mosaic images
    • Automated multi-point imaging
  • PI piezoelectric Z-stage:
    • Fast confocal acquisition of multiple Z-stacks
  • PFS focus stabilization and NIS imaging software

Zeiss AxioObserver Z1 Inverted Widefield Microscope

  • Epifluorescence imaging
    • DAPI, CFP, GFP, YFP, Cy3, Texas Red, Cy5 and Cy7 filters
    • Hamamatsu ORCA Flash 4.0 LT sCMOS monochrome camera
  • Brightfield transmitted light imaging
    • Brightfield, DIC and Phase contrast
    • AxioCam MRc5 color CCD camera
  • CoolLED pE-4000 16-channels fluorescence excitation light source
  • Fully automated scanning stage for tile imaging and Heated stage and chamber system for live cell imaging
  • Definite Focus and Zen image acquisition and analysis software