Instrumentation

NCI Optical Microscopy Laboratory

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Instrumentation

Zeiss LSM 880 NLO Laser Scanning Microscope

Laser scanning microscope:

o 355nm UV laser
o 405nm, 594nm, 561nm and 633nm diode lasers
o Argon laser (458nm, 488nm and 514nm lines)

Coherent Chameleon Vision II tunable (680nm – 1080nm) IR multiphoton laser system
Epifluorescence sample preview:

o DAPI, CFP, GFP, YFP, Rhodamine, and mCherry filters
o Qdot 565, 585, 605, and 655 filters

63x oil-immersion and 40x water-immersion objectives
 Definite focus and Motorized scanning stage
Heated incubation chamber with CO2 and humidity control for live cell imaging
ZEN imaging software with FCS and photobleaching modules

Yokogawa CSU-W1 Spinning Disk Confocal on Nikon T2i Eclipse Microscope

Confocal imaging with 405nm, 488nm, 561nm and 640nm lasers
Epifluorescence wide-filed imaging with Lumencor Sola lightsource:

o DAPI, FITC, Cy3 and Cy5 channels

CoolLED 320/380nm lightsource for Fura-2 calcium imaging
Two Hamamatsu Orca Flash4 sCMOS cameras
Fully automated scanning stage for tile imaging:

o Fast confocal acquisition of large area mosaic images
o Automated multi-point imaging

PI piezoelectric Z-stage:

o Fast confocal acquisition of multiple Z-stacks

PFS focus stabilization and NIS imaging software

Zeiss AxioObserver Z1 Inverted Widefield Microscope

Epifluorescence imaging

o DAPI, CFP, GFP, YFP, Cy3, Texas Red, Cy5 and Cy7 filters
o Hamamatsu ORCA Flash 4.0 LT sCMOS monochrome camera

Brightfield transmitted light imaging

o Brightfield, DIC and Phase contrast
o AxioCam MRc5 color CCD camera

CoolLED pE-4000 16-channels fluorescence excitation light source
Fully automated scanning stage for tile imaging and Heated stage and chamber system for live cell imaging
Definite Focus and Zen image acquisition and analysis software