NCI Optical Microscopy Laboratories

Software & Techniques

Scientific Publications

Publications (2013 – present)

1. Dimitriou, I.D., Lee, K, Akpan, I., Lind, E.F, Barr, V.A., Ohashi, P.S., Samelson, L.E., and Rottapel, R. Timed regulation of 3BP2 induction is critical for sustaining CD8+ T cell expansion and differentiation. Cell Reports. 24:1123-1135. 2018.

2. Balagopalan, L., Yi, J., Nguyen, T., McIntire, K.M., Harned, A.S., Narayan, K., and Samelson, L.E. Plasma membrane LAT activation precedes vesicular recruitment defining two phases of early T-cell activation. Nat Commun. 9:2013-2030. 2018

3. Guittard, G., Dios-Esponera, A., Palmer, D.C., Akpan, I., Barr, V.A., Manna, A., Restifo, N.P., and Samelson, L.E. The Cish SH2 domain is essential for PLC-γ1 regulation in TCR stimulated CD8+ T cells. Sci. Rep. 8:5336-5345. 2018.

4. Greer, Y.E., Porat-Shliom, N., Nagashima, K., Stuelten, C., Crooks, D., Koparde ,V.N., Gilbert, S.F., Islam, C., Ubaldini. A., Ji, Y., Gattinoni, L., Soheilian, F., Wang, X., Hafner, M., Shetty, J., Tran, B., Jailwala, P., Cam, M., Lang, M., Voeller, D., Reinhold, W.C., Rajapakse, V., Pommier, Y., Weigert, R., Linehan, W.M., and Lipkowitz, S. ONC201 kills breast cancer cells in vitro by targeting mitochondria. Oncotarget 9:18454-18479. 2018.

5. Giardino Torchia, M.L., Dutta, D., Mittelstadt, P.R., Guha, J., Gaida, M.M., Fish, K., Barr, V.A., Akpan, I.O., Samelson, L.E., Tagad, H.D., Debnath, S., Miller Jenkins, L.M., Appella, E., and Ashwell, J.D. Intensity and duration of TCR signaling is limited by p38 phosphorylation of ZAP-70T293 and destabilization of the signalosome. Proc. Natl. Acad. Sci. U S A.115:2174-2179. 2018

6. Wang, W., Chen, S., Das, S., Losert, W., and Parent, C.A. Adenylyl cyclase A mRNA localized at the back of cells is actively translated in live chemotaxing Dictyostelium. J. Cell Sci. 4:131-146. 2018.

7. Stuelten, C.H., Lee, R.M., Losert, W., and Parent, C.A. Lysophosphatidic acid regulates the motility of MCF10CA1a breast cancer cell sheets via two opposing signaling pathways. Cell Signal. 45:1-11. 2018.

8. Wang, T., Hamilla, S., Cam, M., Aranda-Espinoza, H., and Mili S. Extracellular matrix stiffness and cell contractility control RNA localization to promote cell migration. Nat. Commun. 8:896-911. 2017.

9. Yi, J., Manna, A., Barr, V.A., Hong, J., Neuman, K.C., and Samelson, L.E. Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM. J. Vis. Exp. Jun 24;(124)

10. Tang, L.Y., Heller, M., Meng, Z., Yu, L.R., Tang, Y., Zhou, M., and Zhang, Y.E. Transforming Growth Factor-β (TGF-β) Directly Activates the JAK1-STAT3 Axis to Induce Hepatic Fibrosis in Coordination with the SMAD Pathway. J. Biol. Chem. 292:4302-4312. 2017.

11. Barr, V.A., Yi, J., and Samelson, L.E. Super-resolution Analysis of TCR-Dependent Signaling: Single-Molecule Localization Microscopy. Methods Mol. Biol. 1584:183-206. 2017

12. Dutta, D., Barr, V.A., Akpan, I., Mittlestadt, P.R., Singha, L.I., Samelson, L.E., and Ashwell, J.D.: Activation-induced recruitment of calcineurin to the T cell receptor positively regulates proximal signaling and effector functions. Nat. Immunol.18:196-204. 2017.

13. Yasuda, K., Clatterbuck-Soper, S.F., Jackrel, M.E., Shorter, J., and Mili, S. FUS inclusions disrupt RNA localization by sequestering kinesin-1 and inhibiting microtubule detyrosination. Cell Biol. 216:1015-1034. 2017.

14. Greer, Y.E., Gao, B., Yang, Y., Nussenzweig, A., and Rubin, J.S. Lack of Casein Kinase 1 Delta Promotes Genomic Instability – The Accumulation of DNA Damage and Down-Regulation of Checkpoint Kinase 1. PLoS One. 12:e0170903. 2017.

15. Das, S., Parker, J.M., Guven, C., Wang, W., Kriebel, P.W., Losert, W., Larson, D.R., and Parent, C.A. Adenylyl cyclase mRNA localizes to the posterior of polarized DICTYOSTELIUM cells during chemotaxis. BMC Cell Biol. 18:23-34. 2017.

16. Barr, V.A., Sherman, E., Yi, J., Akpan, I., Rouquette-Jazdanian, A.K., and Samelson, L.E. Development of nanoscale structure in LAT-based signaling complexes. Cell Sc.129:4548-4562. 2016.

17. Luo, R., Chen, P.W., Wagenbach, M., Jian, X., Jenkins, L., Wordeman, L., and Randazzo, P.A. Direct functional interaction of the kinesin-13 family member kinesin-like protein 2A (Kif2A) and Arf GAP with GTP-binding protein-like, ankyrin repeats and PH domains 1 (AGAP1). J. Biol. Chem. 291:21350-21362. 2016

18. Sherman, E., Barr, V.A., Merrill, R.K., Regan, C.K., Sommers, C.L., and Samelson, L.E. Hierarchical nanostructure and synergy of multimolecular signaling complexes. Nat. Comm. 7:12161. 2016.

19. Chen, P.W., Jian, X., Heissler, S.M., Le, K., Luo, R., Jenkins, L.M., Nagy, A., Moss, J., Sellers, J.R., and Randazzo, P.A. The Arf GTPase-activating Protein, ASAP1, Binds Nonmuscle Myosin 2A to Control Remodeling of the Actomyosin Network. Biol. Chem. 291:7517-7526. 2016.

20. Meena, N.P., Zhu, G., Mittelstadt, P.R., Giardino Torchia, M.L., Pourcelot, M., Arnoult, D., Ashwell, J.D., and Munitic, I. The TBK1-binding domain of optineurin promotes type I interferon responses. FEBS Lett. 590:1498-1508, 2016.

21. Majumdar, R., Tavakoli Tameh, A., and Parent, C.A. Exosomes Mediate LTB4 Release during Neutrophil Chemotaxis. PLoS Biol. 14:e1002336, 2016.

22. Balagopalan, L., Kortum, R.L., Coussens, N.P., Barr, V.A., and Samelson, L.E. The Linker for Activation of T Cells (LAT) Signaling Hub: From Signaling Complexes to Microclusters. J. Biol. Chem. 290:26422-26429. 2015.

23. Hui, K.L., Balagopalan, L., Samelson, L.E. and Upadhyayaa, A. Cytoskeletal forces during signaling activation in Jurkat T-cells. Mol. Biol. Cell. 26: 685–695. 2015.

24. Posokhova, E., Shukla, A., Seaman, S., Volate, S., Hilton, M.B., Wu, B., Morris, H., Swing, D.A., Zhou, M., Zudaire, E., Rubin, J.S., and St. Croix, B. Functions as a WNT7-Specific Co-Activator of Canonical β-Catenin Signaling. Cell Rep. 10:123-130, 2015.

25. Greer, Y.E., Westlake, C.J., Gao, B., Bharti, K., Shiba, Y., Xavier, C.P., Pazour, G.J., Yang, Y, and Rubin, J.S. Casein kinase 1δ functions at the centrosome and Golgi to promote ciliogenesis. Mol. Biol. Cell. 25:1629-40. 2014.

26. Wang, C., Chowdhury, S., Driscoll, M., Parent, C.A., Gupta, S.K., and Losert, W. The interplay of cell-cell and cell-substrate adhesion in collective cell migration. J. R. Soc. Interface.11:20140684, 2014.

27. Chen, P.W., Luo, R., Jian, X., Randazzo, P.A. The Arf6 GTPase-activating proteins ARAP2 and ACAP1 define distinct endosomal compartments that regulate integrin α5β1 traffic. Biol. Chem. 289:30237-48. 2014.

28. Zyezdova, E., Lee, J., El-Khoury, D., Barr, V., Akpan, I., Samelson, L., and Love, P.E.: In vivo functional mapping of the conserved protein domains within murine Themis1. Cell Biol. 92:721-833. 2014

29. McCann CP, Rericha EC, Wang C, Losert W, Parent CA. Dictyostelium cells migrate similarly on surfaces of varying chemical composition. PLoS One. 9:e87981. 2014.

30. Kortum RL, Balagopalan L, Alexander CP, Garcia J, Pinski JM, Merrill RK, Nguyen PH, Li W, Agarwal I, Akpan IO, Sommers CL, Samelson LE. The ability of Sos1 to oligomerize the adaptor protein LAT is separable from its guanine nucleotide exchange activity in vivo. Sci Signal. 6:ra99. 2013.

31. Yasuda K, Zhang H, Loiselle D, Haystead T, Macara IG, Mili S. The RNA-binding protein Fus directs translation of localized mRNAs in APC-RNP granules. J Cell Biol 203:737-46. 2013.

32. Parker J, Sherman E, van de Raa M, van der Meer D, Samelson LE, Losert W. Automatic sorting of point pattern sets using Minkowski functionals. Phys Rev E Stat Nonlin Soft Matter Phys. 88:022720. 2013.