FRET (Fluorescence Resonance Energy Transfer)
FRET is a light microscopy method for detecting protein‐protein interactions within intact cells.
FCS (Fluorescence Correlation Spectroscopy)
Fluorescence correlation spectroscopy (FCS) is a correlation analysis of fluctuation of the fluorescence intensity.
FRAP (Fluorescence Recovery After Photobleaching)
Fluorescence recovery after photobleaching (FRAP) is a method for determining the kinetics of diffusion through tissue or cells.
Deconvolution
The concept of deconvolution is widely used in the techniques of signal processing and image processing.
PALM (Photo-activated Localization Microscopy)
Resolves individual proteins with a resolution down to 20 nm in intact cells, and second-order statistics to study the spatial interactions of the proteins.
IRM (Interference Reflection Microscopy)
Interference reflection microscopy (IRM) is an optical microscopy technique that utilizes polarized light to form an image of an object on a glass surface.