Publications

1. L. Balagopalan, T. Moreno, H. Qin, B. C. Angeles, T. Kondo, J. Yi, K. M. McIntire, N. Alvinez, S. Pallikkuth, M. E. Lee, H. Yamane, A. D. Tran, P. Youkharibache, R. E. Cachau, N. Taylor, L. E. Samelson, “Generation of antitumor chimeric antigen receptors incorporating T cell signaling motifs”, Sci. Signal., 17 eadp8569 (2024).

2. K. Raychaudhuri, R. Rangu, A. Ma, N. Alvinez, A. D. Tran, S. Pallikkuth, K. M. McIntire, J. A. Garvey, J. Yi, L. E. Samelson, “CD28 shapes T cell receptor signaling by regulating ZAP70 activation and Lck dynamics”, BioRxiv (2024). https://doi.org/10.1101/2024.06.27.601067

3. E. M. Rosenberg Jr., X. Jian, O. Soubias, H. Y. Yoon, M. P. Yadav, S. Hammoudeh, S. Pallikkuth, I. Akpan, P. W. Chen, T. K. Maity, L. M. Jenkins, M. E. Yohe, R. A. Byrd, P. A. Randazzo, “The small molecule inhibitor NAV-2729 has a complex target profile including multiple ADP-ribosylation factor regulatory proteins”, J. Biol. Chem., 299(3), 102992 (2023).

4. Barr, V.A., Piao, J., Balagopalan, L., McIntire, K.M., Schoenberg, F.P., and Samelson, L.E. Heterogeneity of Signaling Complex Nanostructure in T Cells Activated via the T Cell Antigen Receptor. Microscopy and Microanalysis, 29, 1503, 2023. https://doi.org/10.1093/micmic/ozad072

5. Nunes-Santos, C.J., Kuehn, H., Boast, B., Hwang, S., Kuhns, D.B., Stoddard, J, Niemela, J.E., Fink, D.L., Pittaluga, S., Abu-Asab, M., Davies, J.S., Barr, V.A., Kawai, T., Delmonte, O.M., Bosticardo, M., Garofalo, M., Carneiro-Sampaio, M., Somech, R., Gharagozlou, M., Parvaneh, N., Samelson, L.E., Fleisher, T.A., Puel, A., Notarangelo, L.D., Boisson, B., Casanova, J.-L., Derfalvi, B., and Rosenzweig, S.D. Inherited ARPC5 deficiency is an actinopathy imparing cell motility and dissecting cytokine signaling. Nat.Commun., 2023, 14(1):3708. doi: 10.1038/s41467-023-39272-0, 2023.

6. Gasparski AN, Moissoglu K, Pallikkuth S, Meydan S, Guydosh NR, Mili S., mRNA location and translation rate determine protein targeting to dual destinations. Mol Cell. 2023 Aug 3;83(15):2726-2738.e9. doi: 10.1016/j.molcel.2023.06.036. Epub 2023 Jul 27. PMID: 37506697

7. Moissoglu K, Lockett SJ, Mili S., Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids, Methods Mol Biol. 2023;2608:263-280. doi: 10.1007/978-1-0716-2887-4_16. PMID: 36653713

8. Chen PW, Maity TK, Jenkins LM, Yohe ME, Byrd RA, Randazzo PA. The small molecule inhibitor NAV-2729 has a complex target profile including multiple ADP-ribosylation factor regulatory proteins. J Biol Chem. 2023 Mar;299(3):102992. doi: 10.1016/j.jbc.2023.102992. Epub 2023 Feb 8. PMID: 36758799; PMCID: PMC10023970. https://www.jbc.org/article/S0021-9258(23)00124-2/fulltext

9. Pedro MP, Lund K, Kang SWS, Chen T, Stuelten CH, Porat-Shliom N, Iglesias-Bartolome R. A GPCR screening in human keratinocytes identifies that the metabolite receptor HCAR3 controls epithelial proliferation, migration, and cellular respiration. bioRxiv [Preprint]. 2023 May 31:2023.05.30.542853. doi: 10.1101/2023.05.30.542853. PMID: 37398171

10. Moriarty RA, Mili S, Stroka KM., RNA localization in confined cells depends on cellular mechanical activity and contributes to confined migration, iScience. 2022 Feb 1;25(2):103845. doi: 10.1016/j.isci.2022.103845. eCollection 2022 Feb 18. PMID: 35198898

11. Gasilina A, Yoon HY, Jian X, Luo R, Randazzo PA. A lysine-rich cluster in the N-BAR domain of ARF GTPase-activating protein ASAP1 is necessary for binding and bundling actin filaments. J Biol Chem. 2022 Mar;298(3):101700. doi: 10.1016/j.jbc.2022.101700. Epub 2022 Feb 8. PMID: 35143843; PMCID: PMC8902617. https://www.jbc.org/article/S0021-9258(22)00140-5/fulltext

12. Yoon HY, Maron BY, Girald-Berlingeri S, Gasilina A, Gollin JC, Jian X, Akpan I, Yohe ME, Randazzo PA, Chen PW. ERK phosphorylation is dependent on cell adhesion in a subset of pediatric sarcoma cell lines. Biochim Biophys Acta Mol Cell Res. 2022 Aug;1869(8):119264. doi: 10.1016/j.bbamcr.2022.119264. Epub 2022 Apr 3. PMID: 35381293. https://doi.org/10.1016/j.bbamcr.2022.119264

13. Yuan Y, Salinas Parra N, Chen Q, Iglesias-Bartolome R. Oncogenic Hedgehog-Smoothened Signaling Depends on YAP1‒TAZ/TEAD Transcription to Restrain Differentiation in Basal Cell Carcinoma. J Invest Dermatol. 2022 Jan;142(1):65-76.e7. doi: 10.1016/j.jid.2021.06.020. Epub 2021 Jul 20. PubMed PMID: 34293352

14. Stuelten CH, Melis N, Subramanian B, Tang Y, Kimicata M, Fisher JP, Weigert R, Zhang YE., Smurf2 Regulates Inflammation and Collagen Processing in Cutaneous Wound Healing through Transforming Growth Factor-β/Smad3 Signaling, Am J Pathol. 2022 Dec;192(12):1699-1711. doi: 10.1016/j.ajpath.2022.08.002. Epub 2022 Sep 3. PubMed PMID: 36063900; PubMed Central PMCID: PMC9765313.

15. Tang LY, Spezia M, Chen T, Shin JH, Wang F, Stappenbeck F, Lebensohn AM, Parhami F, Zhang YE., Oxysterol derivatives Oxy186 and Oxy210 inhibit WNT signaling in non-small cell lung cancer, Cell Biosci. 2022 Jul 30;12(1):119. doi: 10.1186/s13578-022-00857-9. PubMed PMID: 35908024; PubMed Central PMCID: PMC9338492.

16. Balagopalan, L., Raychaudhuri, K., and Samelson, L.E. Microclusters as T cell signaling hubs: structure, kinetics and regulation. Frontiers in Cell and Developmental Biology, doi: 10.3389/fcell.2020.608530, 2021.

17. Pichon X, Moissoglu K, Coleno E, Wang T, Imbert A, Robert MC, Peter M, Chouaib R, Walter T, Mueller F, Zibara K, Bertrand E, Mili S., The kinesin KIF1C transports APC-dependent mRNAs to cell protrusions, RNA. 2021 Dec;27(12):1528-1544. doi: 10.1261/rna.078576.120. Epub 2021 Sep 7. PMID: 34493599

18. Balagopalan, L., Malik, H., McIntire, K., Garvey, J.A., Nguyen, T., Rodriguez-Peña, A.B., and Samelson, L.E. Bypassing ubiquitination enables LAT recycling to the cell surface and enhanced signaling in T cells. PLOS ONE, e0229036.doi: 10.1371/journal.pone.0229036, 2020.

19. Chrisafis G, Wang T, Moissoglu K, Gasparski AN, Ng Y, Weigert R, Lockett SJ, Mili S., Collective cancer cell invasion requires RNA accumulation at the invasive front, Proc Natl Acad Sci U S A. 2020 Nov 3;117(44):27423-27434. doi: 10.1073/pnas.2010872117. Epub 2020 Oct 15. PMID: 33060293

20. Moissoglu K, Stueland M, Gasparski AN, Wang T, Jenkins LM, Hastings ML, Mili S., RNA localization and co-translational interactions control RAB13 GTPase function and cell migration, EMBO J. 2020 Nov 2;39(21):e104958. doi: 10.15252/embj.2020104958. Epub 2020 Sep 18. PMID: 32946136

21. Hoy JJ, Salinas Parra N, Park J, Kuhn S, Iglesias-Bartolome R. Protein kinase A inhibitor proteins (PKIs) divert GPCR-Gαs-cAMP signaling toward EPAC and ERK activation and are involved in tumor growth. FASEB J. 2020 Oct;34(10):13900-13917. doi: 10.1096/fj.202001515R. Epub 2020 Aug 24. PubMed PMID: 32830375

22. Pedro MP, Salinas Parra N, Gutkind JS, Iglesias-Bartolome R. Activation of G-Protein Coupled Receptor-Gαi Signaling Increases Keratinocyte Proliferation and Reduces Differentiation, Leading to Epidermal Hyperplasia. J Invest Dermatol. 2020 Jun;140(6):1195-1203.e3. doi: 10.1016/j.jid.2019.10.012. Epub 2019 Nov 7. PubMed PMID: 31707029

23. Yuan Y, Park J, Feng A, Awasthi P, Wang Z, Chen Q, Iglesias-Bartolome R. YAP1/TAZ-TEAD transcriptional networks maintain skin homeostasis by regulating cell proliferation and limiting KLF4 activity. Nat Commun. 2020 Mar 19;11(1):1472. doi: 10.1038/s41467-020-15301-0. PubMed PMID: 32193376

24. Tang LY, Thomas A, Zhou M, Zhang YE. Phosphorylation of SMURF2 by ATM exerts a negative feedback control of DNA damage response. J Biol Chem. 2020 Dec 25;295(52):18485-18493. doi: 10.1074/jbc.RA120.014179. Epub 2020 Oct 23. PubMed PMID: 33097595; PubMed Central PMCID: PMC9350827.Used Leica SP8 to visualize ATM / Smurf2-flag in proximity ligation assays

25. Dios Esponera, A., Melis, N., Subramanian, B.C., Weigert, R., Samelson, L.E. Pak1 kinase promotes activated T cell trafficking by regulating the expression of CD62L and CCR7. Front. Immunology, 10:370. doi.org/10.3389/fimmu.2019.00370, 2019.

26. Yi, J., Balagopalan, L., Nguyen, T., McIntire, K.M., and Samelson, L.E. TCR microclusters form spatially segregated domains and assemble in discrete, calcium-dependent kinetic steps. Nat. Commun., 10:277. doi.org/10.1038/s41467-018-08064-2, 2019.

27. Moissoglu K, Yasuda K, Wang T, Chrisafis G, Mili S., Translational regulation of protrusion-localized RNAs involves silencing and clustering after transport., Elife. 2019 Jul 10;8:e44752. doi: 10.7554/eLife.44752. PMID: 31290739

28. Stueland M, Wang T, Park HY, Mili S., RDI Calculator: An Analysis Tool to Assess RNA Distributions in Cells, Sci Rep. 2019 Jun 4;9(1):8267. doi: 10.1038/s41598-019-44783-2. PMID: 31164708

29. Gasilina A, Vitali T, Luo R, Jian X, Randazzo PA. The ArfGAP ASAP1 Controls Actin Stress Fiber Organization via Its N-BAR Domain. iScience. 2019 Dec 20;22:166-180. doi: 10.1016/j.isci.2019.11.015. Epub 2019 Nov 14. PMID: 31785555; PMCID: PMC6889188. https://doi.org/10.1016/j.isci.2019.11.015

30. Roy NS, Jian X, Soubias O, Zhai P, Hall JR, Dagher JN, Coussens NP, Jenkins LM, Luo R, Akpan IO, Hall MD, Byrd RA, Yohe ME, Randazzo PA. Interaction of the N terminus of ADP-ribosylation factor with the PH domain of the GTPase-activating protein ASAP1 requires phosphatidylinositol 4,5-bisphosphate. J Biol Chem. 2019 Nov 15;294(46):17354-17370. doi: 10.1074/jbc.RA119.009269. Epub 2019 Oct 6. PMID: 31591270; PMCID: PMC6873180

31. Dimitriou, I.D., Lee, K., Akpan, I., Lind, E.F., Barr, V.A., Ohashi, P.S., Samelson, L.E., and Rottapel, R. Positive feedback regulation through 3BP2 sustains TCR signaling during CD8+ T cell differentiation. Cell Reports 24:1123-1135, doi.org/10.1016/j.celrep.2018.06.075, 2018.

32. Balagopalan, L., Yi, J., Nguyen, T., McIntire, K., Harned, A.S., Narayan, K., and Samelson, L.E. Plasma membrane LAT recruitment precedes vesicular LAT recruitment to reveal two phases of early T cell activation. Nat. Commun. 9:2013, doi.org/10.1038/s41467-018-04419-x, 2018.

33. Guittard, G., Dios-Esponera, A., Palmer, D.C., Akpan, I., Barr, V.A., Manna, A. Restifo, N.P., and Samelson, L.E. The Cish SH2 domain is essential for PLC-1 regulation in TCR stimulated CD8+ T cells. Sci. Rep., 8(1):5336. doi:10.1038/s41598-018-23549-2, 2018.

34. Torchia, M.L.G., Dutta, D., Mittelstadt, P.R., Guha, J., Gaida, M.M., Fish, K., Barr, V.A., Akpan, I. O., Samelson, L.E., Tagad, H., Debnath, S., Jenkins, L., Appella, E, and Ashwell, J.D. The intensity and duration of TCR signaling is limited by p38 phosphorylation of ZAP-70T293 and destabilization of the signalosome. Proc. Natl. Acad. Sci. USA. 115:2175, 2018.

35. Luo R, Chen PW, Kuo JC, Jenkins L, Jian X, Waterman CM, RandazzoARAP2 inhibits Akt independently of its effects on focal adhesions. Biol Cell. 2018 Dec;110(12):257-270. doi: 10.1111/boc.201800044. Epub 2018 Sep 10.PMID: 30144359