Nikon SoRa Spinning Disk
Capabilities:
- Inverted microscope
- Photo-metrics BSI sCMOS camera
- Yokogawa SoRa CSU-W1 spinning disk unit
- Super-resolution, confocal and wide-field imaging modes
- 4x, 10x, 20x and 60x objective lenses
- Mad City Labs 500 mm piezo stage
- Tokai Hit stage top incubator for live cell imaging
Lasers:
- 405 nm Diode laser
- 445 nm Diode laser
- 488 nm Diode laser
- 514 nm Diode laser
- 561 nm Diode laser
- 594 nm Diode laser
- 638 nm Diode laser
Zeiss LSM 880 Airyscan
- Inverted microscope
- GaAsP spectral detector for confocal imaging
- 4 color Airyscan “super-resolution” module
- Airyscan FAST for faster super-resolution imaging
- Lasers: Argon (458, 488, and 514nm), Diode lasers (405, 561, 594nm), and HeNe laser (633nm)
- 2-photon including BiG2 (2 x GaAsP NDD module)
- Environmental chamber, high-resolution motorized scanning stage with Z-piezo insert for fast Z-stack acquisition
Zeiss ELYRA: LSM 780 SIM
- Inverted microscope
- GaAsP spectral detector
- 4 color Structured Illumination (SIM) “super-resolution”
- Lasers: Argon (458, 488, and 514m), Diode 405 and 561nm), and HeNe (633nm)
- Stage top incubator for live cell SIM imaging
- Motorized scanning stage with Z-piezo for z-stack imaging
Leica Stellaris 8 FLIM
- Inverted Leica DMi8 microscope
- 5 HyD detectors (2 HyD S, 2 HyD X, and 1 HyD R detectors)
- HyD X detectors are used for FLIM imaging
- Confocal and FALCON FLIM imaging modes
- TauGating imaging modes
- Galvo and Resonant Scanners
- 405nm and White Light Laser for excitation (445 to 785 nm); Extended wavelength range for multi-channel and spectral confocal imaging
- 5x, 10x, 20x air, 20x multi-immersion, 40x and 63x objective lenses
- Adaptive Focus Control
- Super Z Galvo Stage
- Fluorescence lifetime gated imaging to reduce background autofluorescence and fluorophore separation (Tau-gating, Tau-separation)
- FALCON FILM
Leica Stellaris 5 STED
- Inverted Leica DMi8 microscope
- 4 HyD S detectors and 1 HyD X STED detector for imaging
- Confocal and STED Super-resolution imaging modes (TauSTED)
- 405nm and White Light Laser for excitation (485 to 685 nm) for confocal imaging
- 775nm STED depletion laser
- 10x, 20x and 40x objective lenses
- 100x objective lens for STED imaging
- Adaptive Focus Control
- Super Z Galvo Stage
- Shared microscope with NOB and CCR
- NOB gets 40% dedicated time (Tues, Thurs & Fri afternoons, primarily Chunzhang Yang’s lab)
- Microscope is housed in CCR Microscopy Core (room B114D)
