Instrumentation

NCI Optical Microscopy Laboratory

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Zeiss Z1 Lighsheet Microscope 

• Dual PCO.edge sCMOS cameras
• Pivot scanner with dual-side illumination
• 4-axis sample positioning system
• Lasers: Diode lasers (405, 488, 561, 638nm)
• 5x and 10x illumination objective lenses
• 5x and 20x detection objective lenses for live samples
• 20x detection objective for cleared samples
• Temp module for live cell imaging

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Zeiss LSM 880 Airyscan

• Inverted microscope
• GaAsP spectral detector for confocal imaging
• 4 color Airyscan “super-resolution” module
• Airyscan FAST for faster super-resolution imaging
• Lasers: Argon (458, 488, and 514nm), Diode lasers (405, 561, 594nm), and HeNe laser (633nm)
• 2-photon including BiG2 (2 x GaAsP NDD module)
• Environmental chamber, high-resolution motorized scanning stage with Z-piezo insert for fast Z-stack acquisition

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Zeiss ELYRA: LSM 780 SIM

• Inverted microscope
• GaAsP spectral detector
• 4 color Structured Illumination (SIM) “super-resolution”
• Lasers: Argon (458, 488, and 514m), Diode 405 and 561nm), and HeNe (633nm)
• Stage top incubator for live cell SIM imaging
• Motorized scanning stage with Z-piezo for z-stack imaging

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Zeiss LSM 780

• Inverted microscope
• GaAsP spectral detector
• Lasers: Argon (458, 488, and 514nm), Diode lasers (405, 561, and 594nm), and HeNe laser (633nm)
• FCS and photon counting modes
• Definite focus, scanning stage
• Oko Labs stage top incubator for live cell imaging

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Zeiss LSM 710 NLO

• Inverted microscope
• 3 PMTs, including 32 channel PMT, 1 transmitted light detector
• Lasers: Argon (458, 488, and 514nm), Diode (405 and 561nm), and HeNe laser (633nm)
• 2-photon laser (690-1040nm)
• 2 non-descanned detectors
• Environmental chamber

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