Supported by CCR Office of Science and Technology Resources (OSTR)

Instrumentation

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Image Analysis

Nikon SoRa Spinning Disk

Capabilities:

  • Inverted microscope
  • Photo-metrics BSI sCMOS camera
  • Yokogawa SoRa CSU-W1 spinning disk unit
  • Super-resolution, confocal and wide-field imaging modes
  • 4x, 10x, 20x and 60x objective lenses
  • Mad City Labs 500 mm piezo stage
  • Tokai Hit stage top incubator for live cell imaging

Lasers:

  • 405 nm Diode laser
  • 445 nm Diode laser
  • 488 nm Diode laser
  • 514 nm Diode laser
  • 561 nm Diode laser
  • 594 nm Diode laser
  • 638 nm Diode laser
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Zeiss LSM 880 Airyscan

  • Inverted microscope
  • GaAsP spectral detector for confocal imaging
  • 4 color Airyscan “super-resolution” module
  • Airyscan FAST for faster super-resolution imaging
  • Lasers: Argon (458, 488, and 514nm), Diode lasers (405, 561, 594nm), and HeNe laser (633nm)
  • 2-photon including BiG2 (2 x GaAsP NDD module)
  • Environmental chamber, high-resolution motorized scanning stage with Z-piezo insert for fast Z-stack acquisition
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Zeiss ELYRA: LSM 780 SIM

  • Inverted microscope
  • GaAsP spectral detector
  • 4 color Structured Illumination (SIM) “super-resolution”
  • Lasers: Argon (458, 488, and 514m), Diode 405 and 561nm), and HeNe (633nm)
  • Stage top incubator for live cell SIM imaging
  • Motorized scanning stage with Z-piezo for z-stack imaging
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Leica Stellaris 8 FLIM

  • Inverted Leica DMi8 microscope
  • 5 HyD detectors (2 HyD S, 2 HyD X, and 1 HyD R)
  • Confocal and FALCON FLIM imaging modes
  • TauGating imaging modes
  • Galvo and Resonant Scanners
  • 405nm and White Light Laser for excitation (440 to 790 nm)
  • 5x, 10x, 20x air, 20x multi-immersion, 40x and 63x objective lenses
  • Adaptive Focus Control
  • Super Z Galvo Stage
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Leica Stellaris STED

  • Inverted Leica DMi8 microscope
  • 4 HyD S detectors and 1 HyD X STED detector
  • Confocal and STED Super-resolution imaging modes (TauSTED)
  • 405nm and White Light Laser for excitation (485 to 685 nm)
  • 775nm STED depletion laser
  • 10x, 20x and 40x objective lenses
  • 100x objective lens for STED imaging
  • Adaptive Focus Control
  • Super Z Galvo Stage
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