Instrumentation

Nikon SoRa Spinning Disk

  • Nikon Ti2-E inverted microscope
  • Hamamatsu ORCA Fusion BT sCMOS camera
  • Yokogawa SoRa CSU-W1 spinning disk unit
  • Super-resolution, confocal and widefield fluorescence imaging modes
  • 4x, 10x, 20x, 25x silicone oil, 40x, 60x oil and 100x oil objective lenses
  • Mad City Labs 500 mm piezo stage
  • Tokai Hit stage top incubator for live cell imaging
  • PFS perfect focus control for live cell imaging
  • 7 diode laser lines: 405, 445, 488, 514, 561, 594, and 640 nm

Zeiss LSM 880 Airyscan

  • Zeiss AxioObserver Z1 inverted microscope
  • GaAsP spectral detector for confocal imaging
  • 4 color Airyscan “super-resolution” module
  • Airyscan FAST for faster super-resolution imaging
  • 5x, 10x, 20x, 25x multi-immersion, 40x oil, 40x water, 63x water, 63x oil and 100x oil objective lenses
  • Environmental encloser and Oko Labs stage top incubator for live cell imaging
  • Definite Focus for live cell imaging
  • Motorized stage with z-piezo for fast Z-stack image acquisition
  • 7 laser lines: Argon (458, 488, 514 nm), three solid-state diode laser (405, 561, 594 nm) and one HeNe laser (633 nm)

Leica Stellaris 8 FLIM

  • Leica DMi8 inverted microscope
  • 5 HyD detectors (2 HyD S, 2 HyD X, and 1 HyD R)
    • HyD X detectors are used for FLIM imaging
  • Confocal and FALCON FLIM imaging modes
  • TauGating imaging modes
  • Galvo and Resonant Scanners
  • 5x, 10x, 20x, 20x multi-immersion, 40x and 63x oil, and 63x water immersion objective lenses
  • Oko Labs stage top incubator for live cell imaging
  • Adaptive Focus Control for living cells
  • Super Z Galvo Stage with extended z-range to 1.5 mm
  • Navigator software module for extended field of view tile imaging
  • 405nm diode laser and White Light Laser for excitation (selectable from 445 to 785 nm))
    • Extended wavelength range for multi-channel and spectral confocal imaging
  • FALCON FILM software module for fluorescence lifetime imaging, including FRET and Phasor plot analysis

Leica Stellaris 5 STED

  • Leica inverted DMi8 microscope
  • Confocal and and STED super-resolution, including Tau-STED and STED Xtend, imaging modes
  • 4 HyD S detectors and 1 HyD X detector
    • HyD X detector used for STED (Stimulated Emission Depletion) super-resolution imaging
  • 5x, 10x, 20x, 20x multi-immersion, 40x oil, 63x oil and 100x oil objective lenses
    • 100x oil plan-apochromat white objective lens for STED imaging
  • 405nm diode laser and White Light Laser for excitation (485 to 685 nm) for confocal imaging
  • 775nm STED depletion laser
  • 10x, 20x and 40x objective lenses
  • Tokai Hit stage top incubator for live cell imaging
  • Adaptive Focus Control for live cell imaging
  • Super Z Galvo Stage with extended z-range to 1.5 mm
  • Navigator software module for extended field of view tile imaging