Welcome to the NCI Core Fluorescence Imaging Facility. We are open to everyone within the NCI, and time permitting; we also welcome collaborations with investigators from other NIH institutes or beyond. We operate a suite of state-of-the-art fluorescence microscopes that enable a broad spectrum of fluorescence imaging experiments. We provide expert guidance and troubleshooting in the design, execution, interpretation, and publication of these experiments. We develop new technologies for both imaging and image analysis, and as these technologies mature we provide our users with access to them. We also offer instruction and outreach in current techniques in fluorescence microscopy in the form of classes and lectures to the NCI, NIH and the larger scientific community.
Our expertise runs the gamut from the most basic forms of fluorescence microscopy, such as dual-label immunofluorescence, to the most sophisticated recent forms such as quantitative FRAP analysis and FRET. We also have extensive experience with time-lapse imaging of cells in 2D or 3D and with computational methods for removal of out of focus light (deconvolution). Our expertise comes not only from helping users apply these various advanced techniques but also from our extensive involvement in the development of these technologies.