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• St. Petersburg University – B.S./M.S. (Genetics) – 1973-1979
• St. Petersburg University – Ph.D. (Genetics) – 1979-1979

• FEBS Fellowship (Vrije Univ., Amsterdam, Holland) – 1991
• American Heart Association Fellowship (Washington Univ., St. Louis, MO) – 1993-1995
• NIH/NCI Group Award of Merit (Bethesda, MD) – 2005

• 1979-1984: Graduate student/Biologist, Department of Genetics, St. Petersburg University, Russia. Studied the transfer of cytoplasmic factors by cytoduction in yeast with S. G. Inge-Vechtomov, St. Petersburg University, Russia. Applied genetic methods (micromanipulation and tetrad analysis, mutagenesis, cytoduction).

• 1984-1991: Assistant Professor; Department of Genetics, St. Petersburg University, Russia. Studied the genetics and molecular biology of chromosomal stability in yeast, St. Petersburg University, Russia. Applied genetic methods (micromanipulation and tetrad analysis, mutagenesis) and molecular biology methods (plasmid and strain construction).

• 1992-1999: Post doctorate in the lab of J. Cooper; Washington University Medical School, St. Louis, MO, USA. Studied the dynamics and genetic control of depolarization of actin cytoskeleton. Used methods of molecular biology (sequencing, Northern, Western analysis, plasmid construction, fluorescence microscopy in live cells, deconvolution, immunofluorescence of yeast, mutagenesis). First publication on the application of GFP to studies of the yeast actin cytoskeleton in collaboration with J. Waddle.

• 1999-present: Manager of the Optical Microscopy Core (OMC)/ Biologist; NCI/NIH, Bethesda, MD, USA. Studied the regulation of transcription in yeast, using CUP1 locus as a model in the lab of J. G. McNally. Used methods of deconvolution, live imaging with fluorescent markers, single molecule FISH and FRAP in research. Managed the Optical Microscopy Facility and applied FRET, FRAP, fluorescence imaging and quantification and cell tracking techniques.

• FAES lecturer in FRET and GFP and live imaging microscopy
• Member of ORSAC (Office of Research Support Advisory Committee)• St. Petersburg University – Ph.D. (Genetics) – 1979-1979

• Gordon Conference, Plant and Fungal Cytoskeleton, Andover, NH, 1995
• Light Microscopy Interest Group, NIH, Bethesda, MD, 2003
• Chromatin Interest Group, NIH, Bethesda, MD, 2008
• Transcription Interest Group, NIH, Bethesda, MD, 2008
• Faculty/Post-doctoral Seminar Series, University of South Dakota Sanford School of Medicine, Vermillion, SD, 2008
• Eppley Seminar Series Speaker, Eppley Institute for Cancer Research, University of Nebraska Medical Center , Omaha, NE, 2008
• Light Microscopy Interest Group, NIH, Bethesda, MD, 2013• St. Petersburg University – Ph.D. (Genetics) – 1979-1979

1. MINFLUX: Mazzocca, M., Narducci, D. N., Grosse-Holz, S., Matthias, J., Karpova, T. S., Hansen, A. S. (2026) Chromatin Dynamics are Highly Subdiffusive Across Seven Orders of Magnitude. Nat Struct Mol Biol. 33: 905-914; doi: 10.1038/s41594-026-01807-6.

2. SMT: Bhatt, B., Yi Wei, Y., Pradhan, A. K., Dhakshnamoorthy, J., Zofall, M., Xiao, H., Vijayakumari, D., Jain, S., Folco, H. D., Qi, H., Ball, D. A., Karpova, T. S., Wheeler, D., Wong, J., Grewal, Shiv I. S. (2026) Stress controls heterochromatin inheritance via histone H3 ubiquitylation. Nature. 650: 768-778; doi: 10.1038/s41586-025-09899-8.

3. Immunofluorescence: Sethi, S. C., Shrestha, R. L., Balachandra, V., Hori, T., Corda, L., Ozbun, L., Karpova, T. S., McKinnon, K., Chari, R., Pegoraro, G., Chih-Chien Cheng, K., Giunta, S., Caplen, N. J., Fukagawa, T., Basrai, M. A. (2025) Chromatin remodeling activity of EP400 safeguards chromosomal stability by preventing CENP-A mislocalization. Cell Rep. 44: 116423; doi: 10.1016/j.celrep.2025.116423.

4. SMT: Fettweis, G., Wagh, K., Stavreva, D. A., Jiménez-Panizo, A., Kim, S., Lion, M., Alegre-Martí, A., Rinaldi, L., Johnson, T. A., Krishnamurthy, M., Wang, L., Ball, D. A., Karpova, T. S., Upadhyaya, A., Vertommen, D., Recio, J. F., Estébanez-Perpiñá, E., Dequiedt, F., Hager, G. L. (2025) Transcription factors form a ternary complex with NIPBL/MAU2 to localize cohesin at enhancers. Nucleic Acids Research. 53: gkaf415; doi: 10.1093/nar/gkaf415.

5. FRAP: Nehru, V., Ball, D., Mukherjee, A., Kurotaki, D., Karpova, T. S., Ozato, K. (2025) Live cell analysis of mobility and decay kinetics of the histone variant H3.3. J. Biol. Chem. 301(6): 108557; doi: 10.1016/j.jbc.2025.108557.

6. Immunofluorescence: Balachandra, V., Thomas, M., Shrestha, R. L., Sethi, S. C., Chari, R., Lin, S., Chih-Chien Cheng, K., Karpova, T. S., Caplen, N. J., Basrai, M. A. (2025) Protein Phosphatase 1 Regulatory Subunit PNUTS Prevents CENP-A Mislocalization and Chromosomal Instability. Mol Cell Biol. 45(5): 185-197; doi: 10.1080/10985549.2025.2487010.

7. Immunofluorescence: Sethi, S. C., Shrestha, R. L., Balachandra, V., Durairaj, G., Au, W. C., Nirula, M., Karpova, T. S., Kaiser, P., Basrai, M. A. (2024) β-TrCP-Mediated Proteolysis of Mis18β Prevents Mislocalization of CENP-A and Chromosomal Instability. Mol Cell Biol. 2024: 1-14; doi: 10.1080/10985549.2024.2382445.

8. MINFLUX, SIM: Correll, C. C., Rudloff, U., Schmit, J. D., Ball, D. A., Karpova, T. S., Balzer, E., Dundr, M. (2024) Crossing boundaries of light microscopy resolution discerns novel assemblies in the nucleolus. Histochemistry and Cell Biology. 162(1-2): 161-183; doi: 10.1007/s00418-024-02297-7.

9. Immunofluorescence: Balachandra, V., Shrestha, R. L., Hammond, C. M., Lin, S., Hendriks, I. A., Sethi, S. C., Chen, L., Sevilla, S., Caplen, N. J., Chari, R., Karpova, T. S., McKinnon, K., Todd, M. A., Koparde, V., Cheng, K. C-C., Nielsen, M. L., Groth, A., Basrai, M. A. (2024) DNAJC9 prevents CENP-A mislocalization and chromosomal instability by maintaining the fidelity of histone supply chains. EMBO J. 43(11): 2166-2197; doi: 10.1038/s44318-024-00093-6.

10. MINFLUX: Ahn, J., Zhang, L., Ravishankar, H., Fan, L., Zeng, Y., Kirsch, K. P., Park, J-E., Yun, H-Y., Ghirlando, R., Ma, B., Ball, D. A., Ku, B., Nussinov, R., Karpova, T. S., Kim, S. J., Wang, Y-X., Lee, K. S. (2023) Architectural basis for cylindrical self-assembly governing Plk4-mediated centriole duplication in human cells. Commun Biol.6(1): 712; doi: 10.1038/s42003-023-05067-8.

11. Immunofluorescence: Shrestha, R. L., Balachandra, V., Kim, J. H., Rossi, A., Vadlamani, P., Sethi, S. C., Ozbun, L., Lin, S., Cheng, K. C-C., Chari, R., Karpova, T. S., Pegoraro, G., Foltz, D. R., Caplen, N. J., Basrai, M. A. (2023) Histone H3/H4 chaperone CHAF1B prevents the mislocalization of CENP-A for chromosomal stability. J. Cell Sci. 2023; doi: 10.1242/jcs.260944.

12. SMT, Methods: Ball, D. A., Jalloh, B., Karpova, T. S. (2022) Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics. Int. J. Mol. Sci. 23(24): 15895; doi: 10.3390/ijms232415895.

13. SMT: Patange, S., Ball, D. A., Wan, Y., Karpova, T. S., Girvan, M., Levens, D., Larson, D. R. (2022) MYC amplifies gene expression through global changes in transcription factor dynamics. Cell Reports. 38(4): 110292; doi: 10.1016/j.celrep.2021.110292.

14. Immunofluorescence: Chen, H., Smith, M., Herz, J., Li, T., Hasley, R., Le Saout, C., Zhu, Z., Cheng, J., Gronda, A., Martina, J. A., Irusta, P. M., Karpova, T. S., McGavern, D. B., Catalfamo, M. (2021) The role of protease-activated receptor 1 signaling in CD8 T cell effector functions. iScience. 24(11): 103387; doi: 10.1016/j.isci.2021.103387.

15. SMT: Patange, S., Ball, D. A., Karpova, T. S., Larson, D. R. (2021) Towards a ‘Spot On’ Understanding of Transcription in the Nucleus. J Mol Biol. 433(14): 167016; doi: 10.1016/j.jmb.2021.167016.

16. Immunofluorescence: Shrestha, R., Rossi, A., Wangsa, D., Zaldana, K., Suva, E., Chun, Y., Sanders, C. L., Hogan, A., Difilippantonio, S., Karpova, T. S., Karim, B., Foltz, D. R., Fachinetti, D., Aplan, P., Ried, T., Basrai, M. (2021) CENP-A overexpression promotes aneuploidy with karyotypic heterogeneity. J. Cell Biol. 220: e202007195.

17. Immunofluorescence: Chernova, T. A., Yang, Z., Karpova, T. S., Shanks, J. R., Scherbik, N., Wilkinson, K. D., Chernoff, Y. O. (2020) Aggregation and Prion-Inducing Properties of the G-Protein Gamma Subunit Ste18 are Regulated by Membrane Association. Int J Mol Sci. 21: 5038.

18. Immunofluorescence: Shah, S., Parmiter, D., Constantine, C., Elizalde, P., Naldrett, M., Karpova, T., Choy, J. (2019) Glucose Signaling Is Connected to Chromosome Segregation Through Protein Kinase A Phosphorylation of the Dam1 Kinetochore Subunit in Saccharomyces cerevisiae. Genetics. 211(2): 531-547; doi: 10.1534/genetics.118.301727.

19. Quantification of cells in tissues: Rahman, M. A., McKinnon, K. M., Karpova, T. S., Ball, D. A., Venzon, D. J., Fan, W., Kang, G., Li, Q., Robert-Guroff, M. (2018) Associations of Simian Immunodeficiency Virus (SIV)-Specific Follicular CD8+ T Cells with Other Follicular T Cells Suggest Complex Contributions to SIV Viremia Control. J. Immunol. 200(8): 2714-2726; doi: 10.4049/jimmunol.1701403.

20. SMT: Serebryannyy, L. A., Ball, D. A., Karpova, T. S., Misteli, T. (2019) Single molecule analysis of lamin dynamics. Methods. 157: 56-65; doi: 10.1016/j.ymeth.2018.08.009.

21. SMT and smFISH: Mehta, G. D., Ball, D. A., Eriksson, P. R., Chereji, R. V., Clark, D. J., McNally, J. G., Karpova, T. S. (2018) Single-Molecule Analysis Reveals Linked Cycles of RSC Chromatin Remodeling and Ace1p Transcription Factor Binding in Yeast. Molecular Cell. 72: 875-887.

22. SMT methods: Presman, D., Ball, D., Paakinaho, V., Grimm, J., Lavis, L., Karpova, T., Hager, G. (2017) Quantifying transcription factor binding dynamics at the single-molecule level in live cells. Methods. 123: 76-88; doi: 10.1016/j.ymeth.2017.03.014.

23. FRET and biosensors: Kannan, R., Song, J-K., Karpova, T., Clarke, A., Shivalkar, M., Wang, B., Kotlyanskaya, L., Kuzina, I., Gu, Q., Giniger, E. (2017) The Abl pathway bifurcates to balance Enabled and Rac signaling in axon patterning in Drosophila. Development. 144(3): 487-498.

24. SMT: Ball, D. A., Mehta, G. D., Salomon-Kent, R., Mazza, D., Morisaki, T., Mueller, F., McNally, J. G., Karpova, T. S. (2016) Single Molecule Tracking of Ace1p in Saccharomyces cerevisiae Defines a Characteristic Residence Time for Non-specific Interactions of Transcription Factors with Chromatin. Nucleic Acids Res. 44: e160.

25. FCS, TICS methods: Mazza, D., Stasevich, T. J., Karpova, T. S., McNally, J. G. (2012) Monitoring dynamic binding of chromatin proteins in vivo by fluorescence correlation spectroscopy and temporal image correlation spectroscopy. Methods Mol Biol. 833: 177-200; doi: 10.1007/978-1-61779-477-3_12.

26. FRAP methods: Mueller, F., Karpova, T. S., Mazza, D., McNally, J. G. (2012) Monitoring dynamic binding of chromatin proteins in vivo by fluorescence recovery after photobleaching. Methods Mol Biol. 833: 153-176; doi: 10.1007/978-1-61779-477-3_11.

27. FRET biosensors: Komiya, T., Coxon, A., Park, Y., Chen, W. D., Zajac-Kaye, M., Meltzer, P., Karpova, T., Kaye, F. J. (2010) Enhanced activity of the CREB co-activator Crtc1 in LKB1 null lung cancer. Oncogene. 29: 1672-1680; doi: 10.1038/onc.2009.453.

28. FRAP and Live Imaging: Tatiana S. Karpova, Min J. Kim, Corentin Spriet, Kip Nalley, Tim Stasevich, Zoulika Kherrouche, Laurent Heliot, James G. McNally (2008) Concurrent Fast and Slow Cycling of a Transcriptional Activator at an Endogenous Promoter. Science. 319(5862): 466-469; doi: 10.1126/science.1150559.

29. FRET methods: Karpova, T. S., McNally, J. G. (2006) Detecting protein-protein interactions with CFP-YFP FRET by acceptor photobleaching. Curr Protoc Cytom. Chapter 12: Unit 12.7; doi: 10.1002/0471142956.cy1207s35.

30. FRET: Koga, F., Xu, W., Karpova, T. S., McNally, J. G., Baron, R., Neckers, L. (2006) Hsp90 inhibition transiently activates Src kinase and promotes Src-dependent Akt and Erk activation. Proc Natl Acad Sci U S A.103(30): 11318-11322.

• University at Albany (SUNY) – B.S. (Physics) – 1996-2000
• University of Tennessee Space Institute – M.S./Ph.D. (Physics) – 2000-2006

• Sigma Pi Sigma Physics Honor Society

2000-2006: Graduate Research Assistant, University of Tennessee Space Institute

Training in general physics with an emphasis on optics. Research focused on the development of instrumentation and data-reduction methods to     use the sensitive detection of fluorescence by confocal microscopy as a means of monitoring molecular dynamics.
       • Constructed custom microscope for Single-Molecule Detection and Imaging by Laser-Induced Fluorescence using both epi-illumination   and prism-based total internal reflection (TIRF).
       • Aided in development of data collection and analysis software for Fluorescence Correlation Spectroscopy (FCS).
       • Developed software to a) analyze temporal brightness fluctuations (blinking) of single fluorescent molecules, and b) deconvolve scattered light signal from time-correlated single-photon counting (TCSPC) measurements to extract fluorescence lifetime.

2007-2010: Postdoctoral Associate, Virginia Bioinformatics Institute

Live cell imaging to measure expression of proteins regulating the cell cycle in Saccharomyces cerevisiae, and the characterization of synthetic biology constructs in Escherichia coli.
       • Developed image processing algorithm to automatically identify cells in phase-contrast microscope images, and track changes in their mean and localized fluorescence over time.
       • Trained and mentored undergraduate and high-school students in the use and analysis of microscopy, flow cytometry and bulk fluorescence measurements for investigations of gene expression.

2011-2014: Senior Research Associate, Virginia Bioinformatics Institute

Live- and fixed-cell imaging to study cell cycle regulation in Saccharomyces cerevisiae, with added responsibilities of laboratory management and software development.
         • Developed algorithms to automatically count in hundreds of individual yeast cells the number of mRNA molecules from cell cycle regulating genes tagged by single-molecule fluorescence in situ hybridization (FISH), and detected with fluorescence microscopy.
         • Led the development of GenoSIGHT, an adaptive microscope control system written in MATLAB to automate the characterization of gene expression in live cells.
         • Created models of gene networks using both COPASI and MATLAB’s SimBiology toolbox
         • Directly supervised a team of four undergraduate students as part of the 2011 International Genetically Engineered Machines (iGEM) competition. The goal of the students’ project was to characterize the maturation kinetics of multiple fluorescent proteins in both yeast and bacteria.

2014-present: Staff Scientist, OMC . 

Develops methods of quantification of Single Molecule Tracking, smFIS data modeling. Supports SMT and cell segmentation in tissues. Builds and maintains custom microscopes.

Dr. Ball provides expert advice and support to Core customers on data collection, data analysis and quantification including but not limited to quantitative analysis of high-resolution statistical mapping of the super-resolution images, FRAP, FCS, RICS, Single molecule tracking, Number and Brightness analysis, and Single Molecule FISH quantification. Dr. Ball is an expert in assembling prototype microscopes that are not yet available commercially and that utilize new optical principles and technical design. Currently Dr. Ball develops instrumentation for the Single Molecule Tracking/Super-resolution microscope and TILT lattice

• Kongju National University, Department of Chemistry, Kongju, South Korea, 2006

1. FLIM: Mohamadreza Fazel, Reza Hoseini, Ayush Saurabh, Lance W. Q. Xu, Lorenzo Scipioni, Giulia Tedeschi, Enrico Gratton, Michelle A. Digman, Steve Presse, Resolving fluorescently labeled species using highly multiplexed spectral FLIM, Scientific Reports (2026). DOI: 10.1038/s41598-026-44023-4.

2. DNA-PAINT: J Shepard Bryan IV, Stanimir Asenov Tashev, Mohamadreza Fazel, Michael Scheckenbach, Philip Tinnefeld, Dirk-Peter Herten, Steve Pressé, Bayesian Inference of Binding Kinetics from Fluorescence Time Series, Journal of Physical Chemistry B 129(19), 4670 (2025). DOI: 10.1021/acs.jpcb.5c01180.

3. Molecular Dynamics: Ricky C. Sexton, Mohamadreza Fazel, Maxwell C. Schweiger, Steve Pressé, Oliver Beckstein, Bayesian nonparametric analysis of residence times for protein-lipid interactions in molecular dynamics simulations, Journal of Chemical Theory and Computation 21(8), 4203 (2025). DOI: 10.1021/acs.jctc.4c01522.

4. STORM: Sajjad Khan, Sandeep Pallikkuth, David J. Schodt, Marjolein B. M. Meddens, Hanieh Mazloom-Farsibaf, Michael J. Wester, Sheng Liu, Ellyse Taylor, Mohamadreza Fazel, Farzin Farzam, Keith A. Lidke, A matlab-based instrument control (mic) package for fluorescence imaging, Journal of Open Source Software 10(105), 7275 (2025). DOI: 10.21105/joss.07275.

5. Fluorescence Microscopy: Mohamadreza Fazel, Kristin S. Grussmayer, Boris Ferdman, Aleksandra Radenovic, Yoav Shechtman, Jörg Enderlein, Steve Pressé, Fluorescence Microscopy: a statistics-optics perspective, Reviews of Modern Physics 96(2), 025003 (2024). DOI: 10.1103/RevModPhys.96.025003. (This work was featured on the journal’s cover.)

6. STORM: David J. Shodt, Michael J. Wester, Mohamadreza Fazel, Sajjad Khan, Hanieh Mazloom-Farsibaf, Sandeep Pallikkuth, Farzin Farzam, Eric A. Burns, William K. Kanagy, Derek A. Rinaldi, Elton Jhamba, Sheng Liu, Peter K. Relich, Mark J. Olah, Stanly L. Steinberg, Keith A. Lidke, SMITE: Single Molecule Imaging Toolbox Extraordinaire (MATLAB), Journal of Open Source Software 8(90), 5563 (2023). DOI: 10.21105/joss.05563.

7. FLIM: Mohamadreza Fazel, Sina Jazani, Lorenzo Scipioni, Alexander Vallmitjana, Songning Zhu, Enrico Gratton, Michelle A. Digman, and Steve Pressé, Building Fluorescence Lifetime Maps Photon-by-photon by Leveraging Spatial Correlations, ACS Photonics (2023). bioRxiv, DOI: 10.1101/2022.11.29.518311.

8. FLIM: Mohamadreza Fazel, Alexander Vallmitjana, Lorenzo Scipioni, Enrico Gratton, Michelle A. Digman, Steve Pressé, Fluorescence Lifetime: Beating the IRF and interpulse window, Biophysical Journal 122, 672-683 (2023). DOI: 10.1101/2022.09.08.507224.

9. FRET: Matthew Safar, Ayush Saurabh, Bidyut Sarkar, Mohamadreza Fazel, Kunihiko Ishii, Tahei Tahara, Ioannis Sgouralis, Steve Pressé, Single photon smFRET. III. Application to pulsed illumination, Biophysical Reports 4 (1), 100088 (2023). DOI: 10.1101/2022.07.20.500892. (This work was featured on the journal’s cover and was highlighted on the Biophysical Society website as cornerstone research.)

10. FRET: Ayush Saurabh, Matthew Safar, Mohamadreza Fazel, Ioannis Sgouralis, Steve Pressé, Single photon smFRET. II. Application to continuous illumination, Biophysical Reports 3 (1), 100087 (2022). DOI: 10.1101/2022.07.20.500888. (This work was featured on the journal’s cover and was highlighted on the Biophysical Society website as cornerstone research.)

11. FRET: Ayush Saurabh, Matthew Safar, Ioannis Sgouralis, Mohamadreza Fazel, Steve Pressé, Single photon smFRET. I. theory and conceptual basis, Biophysical Reports 3 (1), 100089 (2022). DOI: 10.1101/2022.07.20.500887. (This work was featured on the journal’s cover and was highlighted on the Biophysical Society website as cornerstone research.)

12. STORM: Mohamadreza Fazel, Michael J. Wester, David J. Schodt, Sebastian Restrepo Cruz, Sebastian Strauss, Florian Schueder, Thomas Schlichthaerle, Jennifer M. Gillette, Diane S. Lidke, Bernd Rieger, Ralf Jungmann, Keith A. Lidke, High-Precision Estimation of Emitter Positions using Bayesian Grouping of Localizations, Nature Communications 13 (1), 7152 (2022). DOI: 10.1038/s41467-022-34894-2.

13. STORM: William K. Kanagy, Cédric Cleyrat, Mohamadreza Fazel, Shayna R. Lucero, Marcel P. Bruchez, Keith A. Lidke, Bridget S. Wilson, and Diane S. Lidke, Docking of Syk to FcεRI is enhanced by Lyn but limited in duration by SHIP1, Molecular Biology of the Cell (2022). DOI: 10.1091/mbc.e21-12-0603.

14. FLIM: Mohamadreza Fazel, Sina Jazani, Lorenzo Scipioni, Alexander Vallmitjana, Enrico Gratton, Michelle A. Digman, and Steve Pressé, High resolution fluorescence lifetime maps from minimal photon counts, ACS Photonics (2022). DOI: 10.1021/acsphotonics.1c01936.

15. STORM: Mohamadreza Fazel, Michael J. Wester, Analysis of super-resolution single molecule localization microscopy data: A tutorial, AIP Advances 12(1):010701 (2022). DOI: 10.1063/5.0069349. (This work was featured as the most downloaded review paper from the journal in 2022.)

16. STORM: Michael J. Wester, Sandeep Pallikkuth, Hanieh Mazloom-Farsibaf, Mohamadreza Fazel, David Schodt and Keith A. Lidke, Robust, fiducial free drift correction for super-resolution imaging, Scientific Reports 11, 23672 (2021). DOI: 10.1038/s41598-021-02850-7.

17. STORM: Hanieh Mazloom-Farsibaf, Farzin Farzam, Mohamadreza Fazel, Michael J. Wester, Marjolein Meddens, Keith A. Lidke, Comparing lifeact and phalloidin for super-resolution imaging of actin in fixed cells, PLoS One 16(1), e0246138 (2021).

18. STORM: Mohamadreza Fazel, Michael J. Wester, Hanieh Mazloom-Farsibaf, Marjolein B. M. Meddens, Alexandra Eklund, Thomas Schlichthaerle, Florian Schueder, Ralf Jungmann, Keith A. Lidke, Bayesian multiple emitter fitting using reversible jump Markov chain Monte Carlo, Scientific Reports 9, 13791 (2019). DOI: 10.1038/s41598-019-50232-x.

19. STORM: Hamidreza Heydarian, Florian Schueder, Maximilian T. Strauss, Ben van Werkhoven, Mohamadreza Fazel, Keith A. Lidke, Ralf Jungmann, Sjoerd Stallinga, Bernd Rieger, Template-free 2D particle fusion in localization microscopy, Nature Methods 15(10) (2018). DOI: 10.1038/s41592-018-0136-6.

• Shahrekord University – B.S. (Physics) – 2006-2010
• Isfahan University of Technology — M.S. (Physics) – 2010-2013
• University of New Mexico – M.S. (Optical Engineering) – 2014-2016
• University of New Mexico – Ph.D. (Physics) – 2016-2020

• Honor student at Shahrekord University for maintaining a high GPA throughout the program, 2010
• Graduate Studies Excellence Assistantship (GSEA), University of New Mexico, 2017
• S-CAP trave; award, University of New Mexico, 2019

2014-2016 graduate teaching assistant, University of New Mexico

• Teaching multiple courses and introductory physics labs

2016-2020 graduate research assistant, University of New Mexico

• Training in a biophysics lab where I worked on the analysis of super-resolution single molecule localization microscopy data.
• Developed a multi-emitter localization method to pinpoint emitters within densely regions with overlapping PSFs and structured backgrounds in SMLM data
• Developed a Bayesian method to achieve nanometer localization precisions using SMLM data
• Involved in developing a comprehensive data processing and post-processing pipeline for SMLM data.
• Involved in the development of a drift correction tool as a postprocessing of SMLM data.

2020-2024 postdoctoral research scholar, Arizona State University

• Developing tools for fluorescent lifetime imaging microscopy to achieve high spatial and lifetime resolutions while learning the number of species present within the data
• Involved in developing a comprehensive methodological framework for FRET data analysis capable of inferring the number of conformational states.
• Developing a 3D tracking frameworks using aberrated PSFs.
• Developing a computational framework for spectral-FLIM data analysis capable of learning dealing with many species with overlapping spectra.

2024-presnt staff scientists, National Cancer Institute, Laboratory of Receptor Biology and Gene Expression

• Developing computational tools for the processing and post-processing of MINFLUX and DNA-PAINT data.

As a staff scientist at the optical microscopy core (OMC) in the Laboratory of Receptor Biology and Gene Expression. He has a strong interdisciplinary background in applying cutting-edge statistical machine learning methods to address problems in biophysics, fluorescence microscopy and optics. At OMC, his research is mainly focused on pattern recognition in data from advanced nanoscopy methods, such as MINFLUX and DNA-PAINT.

Invited Zoom Talk—LFD Workshop, University of California, Irvine, California, USA, November 2023.
Popular Science Talk—Science On Tap, Tempe, AZ, USA, October 2023.
Invited Zoom Talk—Physics Seminar, University of Calgary, Alberta, Canada, January 2022.
Invited Zoom Talk–Physics Colloquium, Isfahan University of Technology, Isfahan, Iran, October 2021.

1. SMT: Bhatt, B., Yi Wei, Y., Pradhan, A. K., Dhakshnamoorthy, J., Zofall, M., Xiao, H., Vijayakumari, D., Jain, S., Folco, H. D., Qi, H., Ball, D. A., Karpova, T. S., Wheeler,D., Wong, J., Grewal, Shiv I. S. Stress controls heterochromatin inheritance via histone H3 ubiquitylation (2026) Nature. 650: 768-778

2. SMT: Fettweis, G., Wagh, K., Stavreva, D. A., Jiménez-Panizo, A., Kim, S., Lion, M., Alegre-Martí, A., Rinaldi, L., Johnson, T. A., Krishnamurthy, M., Wang, L., Ball, D. A., Karpova, T. S., Upadhyaya, A., Vertommen, D., Recio, J. F., Estébanez-Perpiñá, E., Dequiedt, F., Hager, G. L. Transcription factors form a ternary complex with NIPBL/MAU2 to localize cohesin at enhancers (2025) Nucleic Acids Research, 53, gkaf415

3. FRAP: Nehru, V., Ball, D., Mukherjee, A., Kurotaki, D., Karpova, T. S., Ozato, K. Live Cell Analysis of Mobility and Decay Kinetics of the Histone Variant H3.3 (2025) J. Biol. Chem 301(6) 108557

4. MINFLUX, SIM: Correll, C. C., Rudloff, U., Schmit, J. D., Ball, D. A., Karpova, T. S., Balzer, E., Dundr, M., Crossing boundaries of light microscopy resolution discerns novel assemblies in the nucleolus (2024) Histochemistry and Cell Biology. 162(1-2):161-183

5. MINFLUX: Ahn, J., Zhang, L., Ravishankar, H., Fan, L., Zeng, Y., Kirsh, K. P., Park., J-E., Yun, H-Y., Ghirlando, R., Ma, B., Ball, D. A., Ku, B., Nussinov, R., Karpova, T. S., Kim, S. J., Wang, Y-X., and Lee, K. S. Architectural basis for cylindrical self-assembly governing Plk4-mediated centriole duplication in humans (2023) Commun Biol. 6(1):712

6. SMT, Methods: Ball, D. A., Jalloh, B., and Karpova, T. S. Impact of Saccharomyces cerevisiae on the field of single molecule biophysics (2022) Int. J. Mol. Sci 23

7. SMT: Patange, S., Ball, D. A., Karpova, T. S., Girvan, M., Levens, D., and Larson, D. R. MYC amplifies gene expression through global changes in transcription factor dynamics (2022) Cell Reports 38, 110292

8. SMT: Patange, S., Ball, D. A., Karpova, T. S., and Larson, D. R. Towards a ‘spot on’ understanding of transcription in the nucleus (2021) J Mol Biol 433, 167016

9. SMT and smFISH: Mehta G.D., Ball D. A., Eriksson, P. R., Chereji, R. V., Clark D. J., McNally J. G., Karpova, T. S. Single-Molecule analysis reveals linked cycles of RSC chromatin remodeling and Ace1p transcription factor binding in yeast (2018) Molecular Cell 72: 875-887

10. SMT: Serebryannyy, L. A., Ball, D. A., Karpova T. S., Misteli, T. Single molecule analysis of lamin dynamics(2018) Methods, 157:56-65

11. Quantification of cells in tissues: Rahman, M.A., McKinnon, K.M., Karpova T.S., Ball D.A., Venzon, D. J. Fan, W., Kang, G, Li, Q., Robert-Guroff, M. Associations of Simian Immunodeficiency Virus (SIV)-Specific follicular CD8 T cells with Other Follicular T cells suggest complex contributions to SIV Viremia control. (2018) J. Immunol. 200: 2714-2726

12. SMT: Paakinaho, V., Presman DM, Ball D, Schiltz, RL, Levitt, P, Johnson, T, Mazza D., Morisaki T, Karpova TS, Hager GL. Single-Molecule analysis of steroid receptor and cofactor actin in living cells. (2017). Nature Communications 8: 15896

13. SMT: Presman, D., Ball, D., Paakinaho, V., Grimm, J., Lavis, L., Karpova, T., Hager, G.: Quantifying transcription factor dynamics at the single-molecule level in live cells. (2017) Methods 123: 76-88 PMID: 28315485

14. SMT: Ball, D.A., Mehta, G. D., Salomon-Kent, R., Mazza, D., Morisaki, T., Mueller, F., McNally, J.G., Karpova, T. S. Single Molecule Tracking Of Ace1p In Saccharomyces cerevisiae Defines A Characteristic Residence Time For Non-specific Interactions Of Transcription Factors With Chromatin. (2016) Nucleic Acids Res 44

15. Feedback-controlled image acquisition: Ball, D.A., Lux, M.W., Adames, N.R., and Peccoud, J. Adaptive imaging cytometry to estimate parameters of gene networks models in systems and synthetic biology (2014) PLoS ONE. 9 (9): e107087

16. FISH: Ball, D.A., Adames, N.R., Reischmann, N., Barik, D., Franck, C.T., Tyson, J.J., and Peccoud, J. Measurement and modeling of transcriptional noise in the cell cycle regulatory network (2013) Cell Cycle, 12 (19), 3203

17. Live imaging and Modeling: Ball, D.A., Ahn, T.-H., Wang, P., Chen, K.C., Cao, Y., Tyson, J.J., Peccoud, J. and Baumann, W.T. Stochastic exit from mitosis in budding yeast: Model predictions and experimental observations(2011) Cell Cycle, 10 (6), 999

18. Live Imaging: Ball, D.A., Marchand, J., Poulet, M., Baumann, W.T., Chen, K.C., Tyson, J.J., and Peccoud, J. Oscillatory dynamics of cell cycle proteins in single yeast cells analyzed by imaging cytometry (2011) PLoS ONE, 6 (10): e26272

19. Feedback-controlled image acquisition: Ball, D.A., Moody, S.E., and Peccoud, J. Cyto•IQ: An adaptive cytometer for extracting the noisy dynamics of molecular interactions in live cells in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, D.L Farkas, D.V. Nicolau, and R.C. Leif, eds (2010) Proc. SPIE, 7568, 75681D.

20. Single Molecules: Ball, D.A., Shen, G., and Davis, L.M. Single-Molecule Detection with axial flow near micron-sized capillaries (2007) Appl. Opt., 46, 1157-1164

21. FCS: Davis, L.M., Ball, D.A., Williams, P.E., Matayoshi, E.D., and Swift, K.M. Dealing with reduced data acquisition times in fluorescence correlation spectroscopy for HTS applications in Microarrays and Combinatorial Technologies for Biomedical Applications, D. V. Nicolau and R. Raghavachari, eds. (2003) Proc. SPIE, 4966, 117-128

22. FCS: Davis, L.M., Williams, P.E., Ball, D.A., Matayoshi, E.D., and Swift, K.M. Data reduction methods for application of fluorescence correlation spectroscopy to pharmaceutical drug discovery. (2003) Curr. Pharm. Biotech. 4, 451-462

23. Single Molecules: Davis, L.M., Parker, W. C., Ball, D.A., Williams, J.G.K., Bashford, G.R., Sheaff, P., Eckles, R., Lamb, D.T., and Middendorf, L.R., Imaging of single-chromophore molecules in aqueous solution near a fused-silica interface. (2001) Proc. SPIE, 4262, pp. 301-311

1. SMT: Steve Pressé, Mohamadreza Fazel, Zeliha Kilic, “Systems and methods for simultaneous single particle tracking, phase retrieval and PSF reconstruction”, US Patent App. 18/425,056 (2024).