Protocols

HiTIF Protocols

Cell Fixation

PFA Cell Fixation with the Blue Washer

Cell Seeding

Seeding Cells in the imaging plates using the Multidrop Combi

Functional Genomic Screens

RNAi/CRISPR-KO Screen Assay Development SOP

siRNA/sgRNA Spotting

Optimization of reverse transfection conditions for siRNA or sgRNA oligos

siRNA/sgRNA Transfection

Reverse siRNA/sgRNA Transfection Protocol for Users (ThermoFisher Multidrop Combi)