PLEASE, JOIN US AT LMIG ZOOM SEMINAR!
DATE: Tuesday, June 11, 2024 TIME AND LOCATION: 11 am EST, ZOOM (INVITATION BY LMIG LIST SERVER)
Speaker: Dr. Simona Patange (NIST)
Title: “Evaluation of Fluid Force Microscopy (FluidFM) for genome editing in single cells”
Summary:
Genome editing is a rapidly evolving biotechnology with the potential to transform many sectors of industry. To introduce genome editing biomolecules into cells, one must use a reagent delivery method that could either manipulate cells in bulk or at the single-cell level. In this talk, I present our results using the ‘FluidFM OMNIUM,’ a single-cell manipulation technology with applications for genome editing. I give an overview of the U.S. NIST Genome Editing Program and present our data on the OMNIUM measurement capabilities, including the ability to perform targeted single-cell injections, single-cell isolations, and 1-2 picoliter cytoplasmic extractions. Our research with FluidFM technology builds upon guidance from our recent publication (Patange and Maragh 2022) in which we summarize technologies related to research applications where it is advantageous to have controlled reagent delivery and high-resolution data on the distribution of genomic and phenotypic outcomes of individual cells within a population.
Reference: Patange S, Maragh S. Fire Burn and Cauldron Bubble: What Is in Your Genome Editing Brew? ACS Biochemistry 2022, Article ASAP. https://doi.org/10.1021/acs.biochem.2c00431Organizers:
Christian Combs (NHLBI/NIH), Tatiana Karpova (NCI/NIH) and Simona Patange (NIST)