Supported by CCR Office of Science and Technology Resources (OSTR)

Instrumentation

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Zeiss LSM780 Confocal Microscope

• Inverted microscope
• Two PMTs and one 32 channel GaAsP spectral detector for confocal imaging
• 10x, 20x, 40x, 63x, and 100x objective lenses
• Lasers: Argon (458, 488, and 514nm), Diode lasers (405, 561, 594nm), and HeNe laser (633nm)
• Motorized scanning stage
• Stage top incubator for live cell imaging
• Carl Zeiss Zen Black 2012 Acquisition software

Zeiss AxioObservor Z1 Epifluorescence Microscope

• Inverted microscope
• Colibri 7 LED source with 385nm, 430nm, 475nm, 511nm, 555nm, 590nm and 630nm LEDs
• Filter cubes for DAPI, CFP, YFP, GFP, Cy3, mCherry, and Cy5
• Hamamatsu Orca Flash4.0 V3 sCMOS Camera
• 5x, 10x, 20x, 40x, and 63x objective lenses
• Motorized scanning stage
• Carl Zeiss Zen Blue 3.9 Acquisition software

Nikon Eclipse Ti2-E Inverted Microscope

• Inverted microscope
• Lumencor SOLA SE 365 FISH light engine
• Filter cubes for FISH imaging DAPI, Aqua, Green2, Gold, Red2, and Cy5
• Photometrics Prime BSI sCMOS camera
• 4x, 10x, 20x, 40x, 60x and 100x objective lenses
• Motorized scanning stage with Z piezo insert for fast Z stack acquisition
• Tokai Hit stage top incubator for live cell imaging
• Nikon Elements Acquisition software

Two Zeiss AxioScan Z1 Slide Scanning Microscopes

• Fully automated slide scanning microscope able to create brightfield and fluorescent virtual slides
• Colibri 7 LED source with 385nm, 430nm, 475nm, 555nm, 590nm and 630nm, and 730nm LEDs
• Filter cubes for 7-color simultaneous imaging of DAPI, CFP, GFP, Cy3, mCherry, Cy5, and Cy7
• 2.5x, 5x, 10x, 20x, and 40x objective lenses
• Up to 100 1×3 or 50 2×3 slides can be imaged in a single experiment
• Carl Zeiss Zen Blue 3.7 Acquisition software.