Images are acquired on imaging systems controlled by acquisition software often specifically designed for the appropriate microscope. In our Core images are acquired by the following software packages: Softworx (Delta Vision Elite), ZEN (Zeiss confocal microscopes), or Micro-Manager Open Source Microscopy Software (Custom SMT).
The next step, the most important for the scientist, includes image processing (deconvolution, filtering, scaling from 16-bit to 8-bit notation) and measurements for quantification of protein content, FRAP, and FRET. These are achieved on dedicated workstations of the core, utilizing the SoftWorx program (deconvolution), ZEN software (FRET algorithms for sensitized emission and acceptor photobleaching; and FRAP quantification), Metamorph (brightness quantification).
Finally, images may be converted to movies (Quick Time and AVI) on workstations of the Core. Surface rendering is available from Imaris, Arrivis. Deconvolution may be performed by SoftWorx or Huygens. Final preparation of images for publication using programs such as Photoshop or Powerpoint should be done by the user in their own laboratory.