Protocols

NCI Optical Microscopy Laboratory

Microscopy Protocols

FRET (Fluorescence Resonance Energy Transfer)

FRET is a light microscopy method for detecting protein‐protein interactions within intact cells.

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FCS (Fluorescence Correlation Spectroscopy)

Fluorescence correlation spectroscopy (FCS) is a correlation analysis of fluctuation of the fluorescence intensity. 

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FRAP (Fluorescence Recovery After Photobleaching)

Fluorescence recovery after photobleaching (FRAP) is a method for determining the kinetics of diffusion through tissue or cells. 

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Deconvolution

The concept of deconvolution is widely used in the techniques of signal processing and image processing.

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PALM (Photoactivated Localization Microscopy)-Resolving multi-molecular protein interactions

 Resolves individual proteins with a resolution down to 20 nm in intact cells, and second-order statistics to study the spatial interactions of the proteins.

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IRM (Interference Reflection Microscopy)

Interference reflection microscopy (IRM) is an optical microscopy technique that utilizes polarized light to form an image of an object on a glass surface.

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