HiTIF High-Throughput Microscopes
The vast majority of the assays run in the HiTIF are performed either in a 96- or 384-well clear bottom imaging plates. The HiTIF possesses dedicated instruments for automated liquid handling (Fluorescent stains, Immuno-Fluorescence (IF), RNA FISH, or DNA FISH staining), image acquisition and image analysis:
Yokogawa CV8000 High-throughput spinning disk confocal microscope
The Yokogawa CV8000 is HiTIF second high-throughput imaging system. The CV8000 feature set is similar to the CV7000.When compared to the latter, the CV8000 has a newer generation of sCMOS cameras (QE 82%, 2048 X 2048 pixels), and a 40X water objective with higher NA (1.15). The CV8000 is not currently integrated with a robotic arm.
Yokogawa CV7000 microscope with integrated robotic arm and plate hotels for automated plate feeding
The Yokogawa CV7000S high-throughput imaging system is a fully automated dual Nipkow spinning microscope equipped with 4 laser lines (405, 488, 561, 640 nm), IR- and image-based autofocus, 2 large sCMOS cameras (2560 X 2048 pixels) and 6 objectives (4X, 10X, 20X LWD, 20X, 40X and 60X water).The supported plate formats are 6-, 12-, 24-, 48-, 96-, 384-well and sample slides. The CV7000 can image in confocal, epifluorescence, Digital Interference Contrast (DIC) and brightfield mode. The CV7000S includes an integrated environmental chamber which maintains constant temperature, CO2 and humidity for time-lapse, live- cell experiments for up to 72 hrs. In addition the CV7000S is equipped with an integrated liquid dispenser that can transfer reagents from a 96- or 384- well plate into an imaging assay plate during live-cell experiments. Finally, the CV7000 is integrated with a robotic arm, plate hotels, and scheduling software to run automated HTI experiments on up to 42 fixed plates.