Confocal Microscope Image of Fixed Mouse Embryonic Fibroblast (MEF) Cells
The golgi complex (stained red) and the nucleus (stained cyan). The golgi complex modifies, sorts, and packages proteins for cell secretion or use inside the cell. It also plays roles in lipid transport, lysosome creation and proteoglycan synthesis. Samples prepared and imaged by Dr. Luana Scheffer, laboratory o f Dr. Jairaj Achyria, CCR, FNLCR.
3-D Rendering of the Node of a Mouse Embryo
The red staining is F-actin, the green is antibody labeling of acetylated tubulin to visualize cilia, and the cyan is cell nuclei. The sample preparation and labeling were performed in the laboratory of Dr. Terry P. Yamaguchi, Cancer and Developmental Biology Laboratory, Center for Cancer Research, National Cancer Institute. Image acquisition and 3-D rendering were performed in the laboratory of Dr. Stephen Lockett, Advanced Technology Program FNLCR/SAIC-Frederick.
Image of Drosophila Kidney RC Cells
Red channel is antibody-labeled Scrib in RC cells (one of the tumor suppressors in Drosophila) antibody-labeled in Drosophila kidney RC cells; Green is Stat-GFP, which labels the kidney stem cells (RNSC) and transit cells (RB); Blue is DAPI. Sample prepared and images by Dr. Xiankun Zeng, laboratory of Dr. Steven Hou, CCR, FNL and rendered with IMARIS.
Images of Drosophila Prostate
A) Escargot-GFP labeling a subset of cells. B) DAPI nuclear staining. C) Red: phalloidin labeling of F actin. D) Overlay. Sample prepared and imaged by Dr. Shree Ram Singh, Laboratory of Dr. Steven Hou of CCR, NCI-Frederick (unpublished)
Photo bleaching and recovery of GFP STAT1
This movie is an example of fluorescence recovery after photobleaching (FRAP), which is used to analyze the diffusion of proteins in cells. In this example, GFP-STAT1 is in the cytoplasm of live RIMM cells. STAT1 is a member of the Signal Transducers and Activators of Transcription family of transcription factors. The top movie shows photobleaching of GFP in the top left corner of the cell. After a few seconds, fluorescence recovers in the dark region and fluorescence is lost in the unbleached regions by the process of diffusion. Later on the cell’s GFP re-equilibrates. The diffusion coefficient of the protein can be determined from the rate of the recovery. The bottom half of the movie shows differential interference contrast images of the cell, showing that it is alive and appearing healthy.
Collective Cell Migration
The gap closing assay is used for studying collective cell migration. Cells are grown close to confluence in a coverslip bottomed dish with a spacer of approximately 1 mm wide dividing the coverslip into two halves. The experiment is started by removing the spacer. The first frame in the movie shows the dish just after removal of the spacer. Over time the cell migrate into the space. How fast the space fills up with cells measures cell migration.