NCI Optical Microscopy Laboratories

Software & Techniques


General Usage Policies

Principal investigators should download this document, read and sign it.
PI’s should also supply a short written summary of the proposed experiment. If the project changes, PI’s should provide a new or revised summary. After the first 16 hours of usage, PI’s should arrange a progress report meeting with Imaging Core staff. After completion of the project, PI’s are required to supply a short written statement summarizing results of the imaging project, and outlining plans for future presentations and publications of the image data. It is vital for the Core to maintain an up-to-date record of its research contributions for budget justifications and site visits.

Users are expected to be familiar with basic immunostaining and fixation techniques and slide preparation. Users will be trained on the instrument of choice. Given current staffing, all users must learn over a period of several training sessions to become relatively independent.

Users will be given a set of guidelines for Core and instrument usage that is designed to maintain instrument quality and to be fair to other users. These rules must be strictly adhered to, or privileges to use the Core will be revoked.

Due to high demand on these instruments, Core time should be reserved only for imaging needs that cannot be accomplished on standard instruments. Due to high demand users should not sign for more than 4 consecutive hours without approval of the staff.

Acknowledgments to the Facility

For record keeping of the Core research contribution, and to ensure continued proper funding of the Core:
A. Users are requested to notify the director when images collected in the facility are published, or used in presentations at meetings, site visits, etc., and to supply reprints.
B. The Core, including Dr. Karpova and Dr. Ball, should be acknowledged in manuscripts that include data from the Core: “Imaging was performed in CCR/ LRBGE Optical Microscopy Core, and supported by Dr. T.S. Karpova and Dr. D.A. Ball”.

Co-authorship with Core staff
Interactions that involve more than basic instruction on how to operate the microscopes should lead to co-authorship on papers with Dr. Karpova and Dr. Ball. These interactions specifically include projects that involve FRAP, FRET, deconvolution, live cell imaging and colocalization, single molecule tracking and SIM/dSTORM/PALM
By choosing to use our Core you agree to these conditions.

Download LRBGE Policies (MS Word 31KB)