Policies
General Usage Policies
Principal investigators should read this document and sign it. PI’s/postdocs should attach a short-written summary of the project to the signed doc. If the project changes, PIs should provide a new or revised summary. It is vital for the Core to maintain an up-to-date record of its research contributions for budget justifications and site visits.
Users are expected to be familiar with basic immunostaining and fixation techniques and slide preparation. Users will be trained on the instrument of choice. Given current staffing, all users must learn over a period of several training sessions to become relatively independent. Users will be given a set of guidelines for Core and instrument usage that is designed to maintain instrument quality and to be fair to other users. These rules must be strictly adhered to, or privileges to use the Core will be revoked. Due to high demand on these instruments, Core time should be reserved only for imaging needs that cannot be accomplished on standard instruments. Due to high demand users should not sign for more than 4 consecutive hours without approval of the staff.
Users may require assistance on sophisticated instruments, such as SMT HILO scope, Lattice Light Sheet microscope and MINFLUX microscope.
Acknowledgments to the Core
For record keeping of the Core research contribution, and to ensure continued proper funding of the Core:
A. Users are requested to notify the director when images collected in the facility are published, or used in presentations at meetings, site visits, etc., and to supply reprints.
B. The Core, including Dr. Karpova, Dr. Ball, and Dr. Fazel should be acknowledged in manuscripts that include data from the Core: “Imaging was performed in CCR/ LRBGE Optical Microscopy Core, and supported by Dr. T.S. Karpova, Dr. D.A. Ball and by Dr. M. Fazel”.
Co-authorship with Core staff
Interactions involving sophisticated techniques should lead to co-authorship on papers with Dr. Karpova, Dr. Ball, and Dr. Fazel. These interactions specifically include projects that involve MINFLUX, Lattice Light Sheet technique; Single Molecule Tracking; FRAP, FRET. Regarding deconvolution, live cell imaging and colocalization, and SIM/dSTORM/PALM, interactions that involve more than basic instruction also should lead to co-authorship.
By choosing to use our Core you agree to these conditions including co-authorship.
Contact Information: phones, web site and e-mail addresses
Director– Dr. Tatiana Karpova 240-760-6637, karpovat@mail.nih.gov
https://confocal.ccr.cancer.gov/nci-microscopy-core-labs/bethesda/laboratory-of-receptor-biology-and-gene-expression/
The facility is in Building 41, Room C615 and is part of the Laboratory for Receptor Biology and Gene Expression, Dr. Gordon Hager, Chief.